补阳还五汤小鼠体内抗氧化作用的药效动力学研究

Pharmacodynamics study on in vivo anti-oxidant action of Buyang Huanwu Decoction in mice

目的研究补阳还五汤(BHD)小鼠体内的药效动力学过程,为临床合理用药提供参考。方法测定小鼠ig BHD后肝脏组织中丙二醛(MDA)水平和超氧化物歧化酶(SOD)活性,制备BHD含药血清,并观察含药血清对小鼠空白肝匀浆内MDA水平和SOD活性的影响,并进一步考察BHD对MDA及SOD活性影响的时效、量效关系,估算相应的药效动力学参数。结果 BHD整体给药及其含药血清均显著降低小鼠肝内MDA水平,增强SOD的活性,与对照组相比,差异显著(P<0.05)。MDA及SOD的整体和血清药理实验的时-效曲线均呈现明显的多峰现象,相应药效动力学参数除效应达峰时间(tp)和效应呈现半衰期[t1/2(Ka)]比较接近外,其余参数均具有明显差异(P<0.05)。结论 BHD具有显著的抗氧化效应,且药效强度较高,作用时间长,其单次给药的药效动力学模型为一室开放模型。

Objective To study the in vivo pharmacodynamics characteristics of Buyang Huanwu Decoction（BHD） in mice, and to provide reference for rational clinical drug use. Methods The level of MDA and activity of SOD in liver tissue of mice were determined after ig treatment with BHD or its drug-containing serum. Further more, the time-effect and dose-effect relationship of BHD on MDA level and SOD activity were carried out. The parameters of pharmacodynamics were estimated based on the time-effect and dose-effect curves. Results Compared with control group, both BHD and its drug-containing serum could reduce MDAlevel, while raise SOD activity in the liver of mice（P〈0.05）. Time-effect curves both present multi-peak but most pharmacodynamics parameters showed significant differences（P〈0.05）, except tp and t1/2（Ka）. Conclusion BHD has obvious antioxidant effect and pharmacodynamics characteristics can be described as one-compartment model.