一株致香真菌香气成分的GC-MS分析

GC-MS Analysis of the Aroma Compounds Produced by an Aroma Fungi

[目的]GC-MS分析致香真菌香气成分。[方法]用顶空液相微萃取法(HLSM)和有机溶剂萃取法对一株致香真菌产生的香气进行萃取分析。[结果]顶空液相微萃取法,萃取剂甲醇的效果好,最适萃取体积为3μl,萃取时间为5 min。其中烃类有8种,含量占总量的8.03%;醇类有2种,含量占总量的0.03%;酯类有5种,含量占总量的7.95%;酮类为3种,含量占总量的0.81%;酸类有2种,含量占总量的0.11%。有机溶剂萃取法,萃取剂乙醚的萃取效果好,其中烃类有20种,含量占总量的56.20%;酸类3种,含量占总量的3.07%;酯类7种,含量占总量的21.27%;醇类2种,含量占总量的1.62%;酮类有1种,含量占总量的0.51%。[结论]对该致香真菌香气成分的分析可为其应用提供参考。

[Objective] The aroma compounds produced by a fungi were analyzed by GC-MS. [Method] Two methods for extracting sweet smelling substance of an aroma fungi by Head-Space Liquid-Phase Microextraction（ HSLM） and Solvent Extraction. GC-MS was used in the research. [Result] The method of HSLM,better extractant was methanol,extractant volume was 3 μl,time was 5 minutes. Volatile compounds were hydrocarbon（ 8 kinds,taking up relative content 8. 03%）,alcohol（ 2 kinds,taking up relative content 0. 03%）,esters（ 5kinds,taking up relative content 7. 95%）,ketones（ 3 kinds,taking up relative content 0. 81%）,and acids（ 2 kinds,taking up relative content 0. 11%）. The method of Solvent Extraction,better extractant was ether. Volatile compounds were hydrocarbon（ 20 kinds,taking up relative content 56. 20%）,alcohol（ 2 kinds,taking up relative content 1. 62%）,esters（ 7 kinds,taking up relative content 21. 27%）,ketones（ 1 kinds,taking up relative content 0. 51%）,and acids（ 3 kinds,taking up relative content 3. 07%）. [Conclusion]Analysis of aroma components of the aroma fungi laid the foundation for study and development of natural perfume.