重症胰腺炎大鼠血清和胰腺组织Ang-Ⅱ、TNF-α、IL-10水平的变化

Changes of Ang-Ⅱ,TNF-α and IL-10 levels in serum and pancreatic tissue of rats with severe acute pancreatitis

目的:探讨重症急性胰腺炎(SAP)大鼠血清和胰腺组织中血管紧张素Ⅱ(Ang-Ⅱ)、肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)水平的动态变化及相关性。方法:64只健康雄性SD大鼠随机分为假手术组(SO组)和SAP组。利用ELISA法检测1、3、6、12 h血清、胰腺组织匀浆中TNF-α、Ang-Ⅱ、IL-10的水平。结果:SAP组血清、胰腺组织中Ang-Ⅱ水平较SO组明显升高(P<0.05)。SAP组胰腺组织TNF-α水平明显升高,3 h达到高峰,而血清TNF-α水平1 h明显升高,6 h达高峰,与SO组比较,各时点差异具有统计学意义(P<0.05)。SAP组胰腺组织IL-10水平各时点较SO组明显升高(P<0.05);血清IL-10 3 h开始升高,12 h达高峰。结论:Ang-Ⅱ、TNF-α、IL-10在SAP大鼠胰腺组织及血清中平行产生,且均明显升高,表明Ang-Ⅱ参与胰腺炎过程,通过打破促/抗炎失衡,进而加重胰腺炎的炎症反应。

Objective：To investigate the dynamic changes of levels of angiotensin Ⅱ （Ang-Ⅱ）,tumor necrosis factor alpha （TNF-α） and interleukin-10 （IL-10） in serum and pancreatic tissue of rats with severe acute pancreatitis （SAP）.Methods：Sixty-four healthy male SD rats were randomly divided into sham operation group （SO group） and SAP group.Levels of TNF-α,Ang-Ⅱ,IL-10 in serum and pancreatic tissue were observed in 1,3,6 and 12 hours after surgery.Results：Level of Ang-Ⅱ in serum and pancreatic tissue were significantly increased in SAP group than those in SO group （P 〈 0.05）.Level of TNF-α in pancreatic tissue in SAP group significantly increased,and reach peak at 3 hours,while level of serum TNF-α was significantly raised at 1 hour,and reached its peak at 6 hours,and there was statistic difference compared with that of SO group （P 〈 0.05）.Level of IL-10 in pancreatic tissue of SAP group was increased at each time point （P 〈0.05）,but serum level of IL-10 began to increase at 3 hours,and reached peak at 12 hours after surgery.Conclusions：Ang-Ⅱ,TNF-α,IL-10 were parallelly produced and increased in pancreas and serum of rats with SAP.Pro / anti-inflammatory imbalance is the way to aggravate the inflammatory reaction of SAP.