摘要
目的:建立稳定的成年SD大鼠心肌细胞缺氧模型,并对心肌细胞内Ca2+动态变化进行测定。方法:用缺氧液灌流30 min模拟心肌缺氧环境,共聚焦显微镜下测定Ca2+动态变化。结果:缺氧导致心肌细胞静息钙水平升高,钙瞬变峰值降低,钙瞬变上升和下降时程延长,肌质网钙储量下降,钙泵功能降低。结论:缺氧导致心肌细胞钙超载,对心肌细胞钙瞬变有抑制作用。
Objective：To establish a hypoxia model using SD rat cardiomyocytes,and to investigate their calcium dynamics. Methods：Calcium transients were recorded by a confocal calcium imaging system from rat cardiac myocytes which were perfused with hypoxia liquid for 30 minutes to simulate the hypoxia conditions. Results：The hypoxia produced a substantial increase in diastolic[Ca^2＋]iand reduced the amplitude of calcium transients. Both rising time and decay time of Ca^2＋ transients were significantly prolonged. The calcium content of sarcoplasmic reticulum（SR）decrease and function of calcium pump of SR reduce. Conclusion：The hypoxia can lead to cardiac myocytes calcium overload and inhibit calcium transients.
出处
《汕头大学医学院学报》
2014年第4期205-207,F0002,共4页
Journal of Shantou University Medical College
基金
国家自然科学基金面上项目(81473215)
2013年汕头市医疗科技计划资助项目
关键词
缺氧
心肌细胞
钙瞬变
hypoxia
cardiac myocyte
calcium transient
