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Biodegradation Kinetics and Mechanism of a Typical Coal Gasification Pollutant:Quinoline

Biodegradation Kinetics and Mechanism of a Typical Coal Gasification Pollutant:Quinoline
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摘要 A strain Pseudomonas sp.PP1 was isolated from activated sludge and characterized by morphological observation,biochemical and physiological identification as well as 16 S rRNA gene sequence analysis.It was a gram-negative,non-motile,rod-shaped with colonies which were white,opaque,small and wrinkled on solid quinoline-MSM.The strain was negative for catalase,gelatin liquefaction,M.R and V.P tests and was unable to reduce / restore nitrate.The 1406 bp 16 S rRNA gene fragment of PP1 was more than 99% identical to the Pseudomonas sp.Biodegradation of quinoline as single substrate was conducted in batch experiments at different temperatures( 15- 40 ℃) and p H values( 5- 10).The results indicate that the optimum conditions for the degradation of quinoline by PP1 were 30 ℃ and p H 7.Pseudomonas sp.PP1 was able to degrade 97.1%,95.4%,94.8%,63.6% and 40.4% quinoline when initial concentrations of quinoline were 50,100,200,300,and 400 mg / L,respectively,and lag phases were prolonged from 2 h to 10 h.The maximum degradation rate( q) was obtained at an initial quinoline concentration( S0) of about 100 mg/L with q of 0.082.The experimentally obtained q values at various initial S0 were fitted by Haldane model,Yano model,Aiba model,Edward model and Webb model,and the results demonstrated that Haldane model gives the best fit for strain PP1 with coefficient of determination,R2= 0.9124 and SDavg= 0.0113208.Five metabolic intermediates were identified by high performance liquid chromatograph( HPLC) and GC / MS.Finally,a possible pathway containing 5,6-dihydroxy-2-oxo-1,2-dihydroquinoline as an intermediate was proposed for the first time. A strain Pseudomonas sp.PP1 was isolated from activated sludge and characterized by morphological observation,biochemical and physiological identification as well as 16 S rRNA gene sequence analysis.It was a gram-negative,non-motile,rod-shaped with colonies which were white,opaque,small and wrinkled on solid quinoline-MSM.The strain was negative for catalase,gelatin liquefaction,M.R and V.P tests and was unable to reduce / restore nitrate.The 1406 bp 16 S rRNA gene fragment of PP1 was more than 99% identical to the Pseudomonas sp.Biodegradation of quinoline as single substrate was conducted in batch experiments at different temperatures( 15- 40 ℃) and p H values( 5- 10).The results indicate that the optimum conditions for the degradation of quinoline by PP1 were 30 ℃ and p H 7.Pseudomonas sp.PP1 was able to degrade 97.1%,95.4%,94.8%,63.6% and 40.4% quinoline when initial concentrations of quinoline were 50,100,200,300,and 400 mg / L,respectively,and lag phases were prolonged from 2 h to 10 h.The maximum degradation rate( q) was obtained at an initial quinoline concentration( S0) of about 100 mg/L with q of 0.082.The experimentally obtained q values at various initial S0 were fitted by Haldane model,Yano model,Aiba model,Edward model and Webb model,and the results demonstrated that Haldane model gives the best fit for strain PP1 with coefficient of determination,R^2= 0.9124 and SDavg= 0.0113208.Five metabolic intermediates were identified by high performance liquid chromatograph( HPLC) and GC / MS.Finally,a possible pathway containing 5,6-dihydroxy-2-oxo-1,2-dihydroquinoline as an intermediate was proposed for the first time.
出处 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2014年第6期121-128,共8页 哈尔滨工业大学学报(英文版)
关键词 pseudomonas sp. QUINOLINE biodegradation kinetics PATHWAY pseudomonas sp. quinoline biodegradation kinetics pathway
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  • 1Aislabie, J., Bej, A. K., Hurst, H., Rothenburger, S. R. and Atlas, R. M. 1990. Microbial degradation of quinoline and methylquinolines. Appl. Environ. Microbiol. 56: 345-351.
  • 2Astruc, D. 2002. Modern Aroma Chemistry: Concepts, Synthe?sis, and Applications. Wiley-VCH, Weinheim. Blaschke, M., Kretzer, A., Schafer, C., Nagel, M. and Andreesen, J. R. 1991. Molybdenum-dependent degradation of quino?line by Pseudomonas putida Chin IK and other aerobic bac?teria. Arch. Microbiol. 155: 164-169.
  • 3Bond, D. R. and Lovley, D. R. 2003. Electricity production by Geobacter sulfurreducens attached to electrodes. Appl. Environ. Microbiol. 69: 1548-1555.
  • 4Catal, T., Fan, Y. Z., Li, K. C., Bermek, H. K. and Liu, H. 2008. Effects of fur an derivatives and phenolic compounds on electricity generation in microbial fuel cells. J. Power Sources. 180: 162-166.
  • 5Fetzner, S. 1998. Bacterial degradation of pyridine, indole, quinoline and their derivatives under different redox con?ditions. Appl. Microbiol. Biot. 49: 237-250.
  • 6Freguia, S., Masuda, M., Tsujimura, M. and Kano, K. 2009. Lac?tococcus lactis catalyses electricity generation at microbial fuel cell anodes via excretion of a soluble quinone. Bioelec?trochemistry. 76: 14-18.
  • 7Gorby, Y. A., Yanina, S., Mclean, J. S., Rosso, K. M., Moyles, D., Dohnalkova, A., Beveridge, T. J., Chang, 1. S., Kim, B. H., Kim, K. S., Culley, D. E., Reed, S. B., Romine, M. F., Saf?farini, D. A., Hill, E. A., Shi, L., Elias, D. A., Kennedy, D. W., Pinchuk, G., Watanabe, K., Ishii, S., Logan, B., Neal?son, K. H. and Fredrickson, J. K. 2006. Electrically conduc?tive bacterial nanowires produced by Shewanella oneidensis strain MR~l and other microorganisms. P. Natl. Acad. Sci. USA. 103: 11358-11363.
  • 8Kargi, F., Eker, S. and Uygur, A. 2005. Biological treatment of synthetic wastewater containing 2,4-dichlorophenol (DCP) in an activated sludge unit. J. Environ. Manage. 76: 191- 196.
  • 9Kim, B. H., ~.im, H. J., Hyun, M. S. and Park, D. H. 1999. Direct electrodi~eaction of Fe (III) reducing bacterium, Shewanella putrefaciens. J. Microbiol. Biotechnol. 9: 127-131.
  • 10Lee, J., Phung, N. T., Chang, 1. S., Kim, B. H. and Sung, H. C. 2003. Use of acetate for enrichment of electrochemically active microorganisms and their 16S rDNA analyses. FEMS Microbiol. Lett. 223: 185-191.

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