北京市2012年常住人口百日咳抗体水平调查

Survey of Antibodies to Pertussis in Resident Population of Beijing in 2012

目的了解北京市2012年常住人口百日咳抗体水平。方法在北京市9个区（县）选择连续居住≥6个月的10个年龄组人群作为调查对象,共2147人。采用问卷调查收集调查对象的人口学特征、含百日咳成分疫苗（Pertussis-containing Vaccine,PCV）接种史、百日咳患病史。采集静脉血4-5毫升（ml）,用酶联免疫吸附试验检测百日咳毒素免疫球蛋白G。结果百日咳抗体阳性率为12.34%（265/2147）,抗体浓度中位数为5.029国际单位（International Unit,IU）/ml。不同户籍、不同免疫史、不同患病史、不同区（县）百日咳抗体差异均无统计学意义。〈1岁婴儿抗体阳性率和抗体浓度最高,随后明显下降,差异有统计学意义（χ2=31.293,P〈0.001;H=99.632,P〈0.001）。〈15岁儿童99.30%（853/859）有PCV接种史,不同剂次PCV免疫的百日咳抗体阳性率和抗体浓度差异有统计学意义（χ2=11.339,P=0.003;H=61.043,P〈0.001）,1-14岁各年龄组PCV不同免疫剂次的百日咳抗体浓度和抗体阳性率差异均无统计学意义。≥10岁调查对象抗体浓度〉100 IU/ml比例为1.33%（20/1504）,计算≥10岁人群百日咳感染率为8.29%。结论北京市常住人口对百日咳普遍易感。

Objective To investigate the level of antibodies against pertussis among resident population of Beijing in 2012. Methods A total of 2147 subjects from 10 age groups, who had been living in Beijing for at least 6 months, were selected from 9 districts of Beijing. Demographic characteristics, history of pertussis infection and vaccine immunization were collected by questionnaire. 5 ml blood sample was col lected from each subject and pertussis toxin （PT） lmmunoglobulin G antibody （AntiFF） was measured by enzymelinked immunosorbent assay. Results Positivity tbr AntiFr was 12.34% （265/2147） ,the median of antibody concentration was 5. 029 International Unit （IU）/ml. There were no significant differences observed in the level of antibodies for population of different household register, history of vac cination, history of pertussis infection, or different districts. Positivity and concentration of antibody were highest among infants aged less than l year and decreased with age, and the differences among age groups were statistically significant （X2 = 31. 293,P 〈 0. 001 ;H = 99. 632, P 〈 0. 001 ）. 99.30% （853/859） of children less than 15 years old had the confirmed history of pertussis vaccination and the differences of antibody concentration and positivity rate of AntiPT were statistically significant （X2 = 13. 412, P = 0.001;H =70.303,P 〈0 001 ） among population with different doses of vaccination. There was no significant differences in the level and positivity of AntiPT among population with different doses of vaccination within each age group of 1 15 yearsold. The proportion of subjects with more than 1.33% （20/1504）, the estimated infection rate 8.29%. Conclusion The population in Beijing 100IU/ml in population aged more than 10 years was of pertussis for population aged more than 10 years was are generally vulnerable to pertussis.