低温胁迫对草菇菌丝体糖原磷酸化酶基因表达的影响

Differential Expression of a Glycogen Phosphorylase Gene in Volvariella volvacea Mycelium Exposed to Low Temperature Stress

在0℃低温胁迫下比较低温敏感型草菇(Volvariella volvacea)菌株V23和耐低温型菌株VH3糖原磷酸化酶(glycogen phosphorylase)pyg基因的表达情况。以α-微管蛋白为内参基因,采用实时荧光定量PCR技术测定pyg基因的表达,结果表明:低温处理4h时,V23的pyg基因相对表达量仅为0h的0.49倍,低温处理6h时有所回升,但仍低于处理0h的水平。VH3的pyg基因相对表达量从低温处理2h时急剧下降,至低温处理8h时达到最低值,仅为0h的0.28倍。随着低温处理时间的延长,pyg基因的相对表达量在V23和VH3中均呈下降趋势,相同的低温处理时间下,该基因在菌株V23中的下降幅度小于VH3中。

Relative expression of a glycogen phosphorylase gene （pyg） in mycelia of cold-sensitive （V23） and cold-tolerant （VH3） strains of Volvariella volvacea during exposure to low temperature （0 ℃） over time courses was quantified by real-time PCR using the a-tubulin gene as internal control. Pyg expression levels in strain V23 decreased after 4 h exposure and, although recovering after 6 h, still remained lower than untreated controls. Gene expression in strain VH3 decreased sharply after low temperature exposure for 2 h, reaching a minimum value after 8 h when the relative expression level was only 0.28 times that of untreated controls. Overall, although pyg expression decreased in both V23 and VH3 over prolonged exposure, the fall was less pronounced in strain V23 compared with VH3.