染铅对大鼠脾脏NOS、NO、SOD、MDA的影响

Effects of Lead Exposure on NOS,NO,SOD,and MDA in Rats Spleen

目的 探讨染铅对大鼠脾脏一氧化氮合酶 (NOS)活力、一氧化氮 (NO)含量的影响及其与血铅相互关系。方法 Wistar大鼠经饮水 (加 0 ,18 4 ,184 0mg/L醋酸铅 ,分别供对照组、低剂量组、高剂量组自由饮用 )染毒 ,测定脾脏NOS活力、NO含量、SOD活力、MDA含量。结果 NOS活力高剂量染铅组 7d时显著低于对照组和低剂量组 (P <0 0 5 ) ,90d高于对照组和低剂量组 (P <0 0 5 ) ,低剂量组 90d高于对照组 (P <0 0 5 ) ;NO含量高剂量组和低剂量组 6 0d、 90d时均显著低于对照组 (P <0 0 5 ) ;SOD活力高剂量组 14d、 30d、 6 0d、 90d时均低于对照组 (P <0 0 5 ) ,低剂量组 30d、 90d时显著低于对照组(P <0 0 5 ) ;MDA含量高剂量组 14d、 6 0d、 90d时均显著高于对照组 ,6 0d时显著低于低剂量组 ,90d时显著高于低剂量组 (P <0 0 5 ) ,低剂量组在各时点与对照组比较 ,差异无显著性 (P >0 0 5 )。血铅与脾脏NO含量呈倒S型剂量 -效应曲线 ;血铅与脾脏SOD活力呈倒抛物线型剂量 -效应曲线 ;血铅与脾脏MDA含量呈正抛物线型剂量 -效应曲线。结论 铅可引起大鼠脾脏NOS活力、NO含量、SOD活力、MDA含量改变 ,表明铅可引起体内氧应激反应导致脂质过氧化损伤。

Objective To study effects of the lead exposure on activity of nitric oxide synthase(NOS)and concentration level of nitric oxide(NO)in rats spleen and their correlation with the blood lead.Methods Wistar rats were exposed to lead by drinking 0,18 4,and 184 mg/L lead acetate solution as the control group,and low and high dose group respectively.Activities of NOS and SOD,and concentration levels of NO and MDA were measured.Results Compared with the control group and low dose group,spleen NOS activity in high dose group lowered significantly at the time of 7 d (P<0 05) ,but became significantly higher at 90 d (P<0 05) .At 90 d ,spleen NOS activity in low dose group showed higher (P<0 05) .As to spleen NO level,those in high and low dose groups were significantly higher than in the control group at 60 and 90 d (P<0 05) .SOD activity in high dose group lowered at 14,30,60 and 90 d (P<0 05) ,and the same results showed in low dose group at 30 and 90 d .MDA level in high dose group was remarkably increased at 14,60 and 90 d (P<0 05) compared with the control group,but significantly lower than in low dose group at the time of 60 d (P<0 05) .Parabola type,converse S type and converse parabola type dose effect curves existed for the relationship between the blood lead concentration and spleen MDA,NO level and SOD activity.Conclusions It indicated that activities of NOS and SOD,and the concentration levels of NO and MDA in rats spleen could be changed after lead exposure causing lipid per oxidization damage.