猪SOCS7基因克隆、组织表达图谱分析及剪接变体鉴定

Characterization of SOCS7 (suppressor of cytokine signaling 7) gene in pigs:cloning,tissue expression,and identification of its alternative splicing variants

以家猪肌肉来源cDNA为模板,克隆了SOCS7的编码区cDNA全长并发现了三种剪接变体(命名为:SOCS7-v1、SOCS7-v2和SOCS7-v3).家猪SOCS7编码区cDNA全长为1785bp,由9个外显子组成,编码含581个氨基酸残基的蛋白质.家猪SOCS7基因的三种剪接变体分别编码含547、520、512个氨基酸残基的蛋白质.通过RT-PCR方法,本研究对家猪SOCS7基因及其剪接变体的组织表达图谱进行了分析,结果显示:SOCS7在精巢、小脑、背肌、腹肌、股肌、大脑、下丘脑、心脏、肝脏、脾脏、小肠等组织中均有表达,在精巢中表达信号最强.SOCS7-v1和SOCS7-v2除大脑外的19个检测组织中均能检测到较强的表达信号,SOCS7-v3在除精巢外的19个组织中均有表达.以上实验结果为探究家猪SOCS7基因的生理功能奠定了基础.

The full-length cDNA of SOCS7 was cloned using pig muscle cDNA as a template,and identi-fied three novel transcript variants （named SOCS7-v1 ,SOCS7-v2 and SOCS7-v3 ）.The full-length cD-NA of SOCS7 encodes a protein of 581 amino acids,while the three novel transcript variants （SOCS7-v1,SOCS7-v2 and SOCS7-v3）encode proteins of 547,521,512 amino acids respectively.Using RT-PCR method,we further analyzed the tissue distribution of SOCS7 and its transcript variants.The re-sults showed that：the full-length SOCS7 protein is expressed in the testis,cerebellum,back muscles, abdominal muscles,femoral muscle,brain,hypothalamus,heart,liver,spleen and small intestine. While the SOCS7-v1 and SOCS7-v2 are expressed in 1 9 tissues except cerebrum;SOCS7-v3 is also ex-pressed in 1 9 tissue examined except testis.These results lay the molecular basis for further exploration of physiological roles of SOCS7 in pigs.