氯化镉对小鼠胚胎神经干细胞迁移的影响

Effect of cadmium chloride on immigration of mouse neural stem cell

目的探讨氯化镉对小鼠胚胎神经干细胞(m NSC)细胞活性和迁移能力的影响。方法建立小鼠神经干细胞体外模型,以0、0.1、0.3、1.0、3.0和10.0μmol/L的氯化镉作用于小鼠胚胎神经干细胞24 h,噻唑蓝(MTT)比色法测定细胞活力;免疫荧光细胞化学法分析细胞迁移。结果 10.0μmol/L的Cd Cl2作用24 h后细胞存活率(70.08±6.21)%显著低于正常对照组(P<0.05);在设定剂量下,氯化镉作用神经干细胞24 h后,Aa/Ab和Dm/Db呈下降趋势,存在剂量依赖性(rs Aa/Ab=-0.90,rs Dm/Db=-0.90,P<0.05)。结论氯化镉对神经干细胞的迁移和活力均有影响,且在同一浓度和相同作用时间下对神经干细胞的迁移影响大于对细胞活力的影响。

Objective To investigate the effects of cadmium chloride on cytoactive and immigration of mouse neural stem cell （ mNSC）. Methods MTT assay was used to detect cytoaetive at 24 hours. The immigration of mNSC was determined by immuno- fluorescence staining. Results Compared with control, CdC12 treatment at 10.0 ptmol/L for 24 h resulted in a decrease in cellular viability（70.08 _＋ 6.21）% （P 〈 0.05）. Compared with control, Aa/Ab and Dm/Db display decreasing tendency in a dose- dependent manner（r Aa/Ab = - 0. 90, r Dm/DI, = - 0. 90, P 〈 0.05 ） after CdC12 treatment at 0. 1 - 10.0 μ mol/L for 24 h. Conclusion Cadmium chloride treatment inhibits immigration of mNSC, and shows negative effect on cell viability. Meanwhile, the effect of cadmium chloride on immigration is more obvious than cell viability at the same concentration for same treatment time.