期刊文献+

酶切法鉴定质粒的亚型 被引量:1

下载PDF
导出
摘要 商品化质粒小量抽提试剂盒制备的质粒DNA,用琼脂糖凝胶电泳检测可见2条亮带,分别回收2条亮带,使用限制性内切酶分别消化,再用琼脂糖凝胶电泳检测,依据质粒的亚型(超螺旋、线性、开环)在以琼脂糖凝胶为介质的电场中的移动速度不同,鉴定出制备的质粒DNA电泳图中的2条亮带分别是超螺旋质粒和开环质粒。
作者 金磊
出处 《生物学通报》 2015年第1期48-49,共2页 Bulletin of Biology
基金 江苏省高校自然科学研究面上项目(16621201)
关键词 质粒 超螺旋 开环
  • 相关文献

参考文献8

  • 1唐瑶云,肖健云,赵素萍,唐爱发,夏昆,陈主初.靶向性质粒表达载体pcDNA3.1(-)CMV·Egr-1CDglyTK的构建及转染研究[J].中国耳鼻咽喉颅底外科杂志,2003,9(1):34-37. 被引量:11
  • 2彭艳,李平,刘苹,周元国.基因治疗用pUC118-ski质粒DNA纯化工艺的建立及其质量控制[J].中国生物制品学杂志,2013,26(10):1483-1487. 被引量:4
  • 3J. C. Wang. Cellular roles of DNA topoisomerases: A molecular perspective, Nat. Rev. Mol. Cell Biology, 2002, (3):430-440.
  • 4David R., Latulippe Andrew L., Zydney: Separation of plasmid DNA isoforms by highly converging flow through small memb- rane pores. Journal of Colloid and Interface Science, 2011, (357):548-553.
  • 5PromegaWizardplusSV小量制备纯化系统技术手册www.promega.com.
  • 6生工生物.SanPrep柱式DNA胶回收试剂盒操作手册.www.sangon.corn.
  • 7S. B. Yu, J. Geng, P. Zhou, etal. Analysis of plasmid DNA damage induced by melanin with capillary electrophoresis. Journal of Pharmaceutial and Biomedical Analysis, 2007, (43): 816-821.
  • 8J. Flemming, J. Arnhold. Ferrous ion-induced strand breaks in the DNA plasmid pBR322 are not mediated by hydrogen peroxide, European Biophysics Journal, 2007,(36):377-384.

二级参考文献12

  • 1Kawashita Y;Ohtsuru A;Kaneda Y.Regression of hepatocellular carcinoma in vitro and in vivo by radiosensitizing suicide gene therapy under the inducible and spatial control of radiation[J],1999(09).
  • 2Datta R;Rubin E;Sukhatme V.Ionizing radiation activates transcription of the EGR1 gene via CArG elsments,1992(21).
  • 3Wei DY;Dai BB;Chen SS.The expression of adenoviral mediated gfp reporter gene in tumor cells induced and regulated by irradiation[J]Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica,2001(01).
  • 4Weichselbam RR;Hallahan DE;Sukhatme VP.Gene therapy targeted by conizing radiation,1992(03).
  • 5Tsai-Morris CH;Cao X;Sukhatme YP.5'flanking sequence and genmic structure of Egr1, a murine mitogen inducible zine finger encoding gene[J],1988.
  • 6Kenneth R;Rogulski;Jae ho Kim.Glioma Cells Transduced with an Escherichia coli CD/HSV-1 TK Fusion Gene Exhibit Enhanced Metabolic Suicide and Radiosensitivity[J],1997(01).
  • 7Staba MJ;Mauceri HJ;Kufe DW.Adenoviral TNE-alpha gene therapy and radiation damage tumor vasculature in a human malignant glioma xenograft[J],1998(03).
  • 8Marples B;Scott SD;Hendry JH.Development of synthetic promoters for radiation-mediated gene therapy[J],2000(06).
  • 9Marples B;Greco O;Joiner MC.Molecular approaches to chemo-radiotherapy[J],2002(02).
  • 10彭艳,李平,刘苹,陈磊,赵晓光,周元国.人ski基因真核表达载体的构建及促细胞增殖作用[J].中国组织工程研究与临床康复,2011,15(41):7693-7696. 被引量:2

共引文献13

同被引文献13

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部