基于微流控芯片的圆褐固氮菌浓度快速检测方法

Rapid Detection of Azotobacter chroococcum Concentration Based on Microfluidic Chip

针对当前微生物肥料中圆褐固氮菌浓度检测方法耗时长、自动化程度低等缺点,提出了一种基于微流控芯片的圆褐固氮菌浓度检测方法。设计了具有自动进样、混合稀释以及电化学循环伏安检测等功能的微流控芯片,构建了微流控检测系统实验平台,优化了系统中的进样速度、扫描速率等工艺参数,并对检测系统的性能指标进行了实验验证。结果表明,与国标平板计数法相比,所提出方法不仅能够满足微生物肥料检测的精度要求,而且具备较快的检测速度及较高的检测自动化程度。

Aiming at the problem that the concentration detection of Azotobacter chroococcum in microbial fertilizer suffers from time-consuming and low automation level, a detecting method of Azotobacter chroococcum concentration based on mierofluidic chip was proposed. A dedicated microfluidic chip with automatic sample injecting, dilution and cyclic vohammetry detection was designed, and the experiment platform based on microfluidic detection system was bulit. The optimum scan rate and feed rate of concentration detection in the microfluidic detection system were determined, and the performance index of the system was experimentally verified. Experiment results showed that, compared with plate counting method, the proposed method met the requirement of microbial fertilizer detection and realized rapid detection with increased automation.