湖北晒晾烟地方种质对烟草白粉病抗性的温室鉴定

Resistance Evaluation of Hubei Origin Air-cured and Sun-cured Tobaccos to Powdery Mildew Evaluated in Greenhouse

为了明确湖北晒晾烟地方种质对烟草白粉病的抗性,利用中国农业科学院烟草研究所保存的白粉病菌,在温室中采用人工喷雾接种方法对湖北的88份地方晒晾烟种质进行了鉴定。结果表明:不同晒晾烟种质对烟草白粉病的抗病性差异明显,88份种质材料中高抗13份,中抗39份,中感和高感均为18份。抗性最高的种质为LC04,ZX06和WF04,其相对抗病指数为0.99;抗性最差的FJY01和CY08,其相对抗病指数不足0.1。对不同抗病类型种质的抗病性差异显著性分析显示,不同抗病类型种质的病叶率、病情指数和相对病情指数均达到显著水平。利用DPS数据分析软件对88份种质进行聚类分析显示,在种质抗性反应谱系图上划分为高抗、中抗、中感和高感4类。

In order to clarify the resistance of Hubei origin germplasms of air-cured and sun-cured tobaccos to powdery mildew, 88 germplasms of air-cured and sun-cured tobacco were evaluated in greenhouse by spraying inoculation with the fungal isolate kept by Tobacco Research Institute of Chinese Academy of Agricultural Sciences. The results showed that the resistance to powdery mildew differed significantly, 13 were highly resistant （HR）, 39 moderately resistant （MR）, 18 moderately susceptible （MS）, and 18 highly susceptible （HS） among the total 88 germplasms. The highest resistant ones were LC04, ZX06 and WF04, with relative resistant indexes of 0.99. The highest susceptible ones were FJY01 and CY08, with relative resistant indexes of less than 0.1. The results of variance analysis indicated that the differences of infected leaf ratio, disease index and relative resistant index reached significantly levels （p〈0.05） between different resistant types. The 88 germplasms were clustered into HR, MR, MS and HS groups by DPS data analyzing software.