卷烟烟气丙烯酰胺暴露量的生物标志物分析及与滤嘴分析法的比较

Assessment of Acrylamide Exposure During Cigarette Smoking by Biomarker Analysis and Comparison with Filter Analysis Method

为了准确评估吸烟过程中人体丙烯酰胺暴露量，建立了测定丙烯酰胺巯基尿酸（MA）代谢物N-乙酰基-S-（2-氨基甲酰乙基）-L-巯基丙氨酸（AAMA）、N-乙酰基-S-（2-氨基甲酰-2-羟基乙基）-L-巯基丙氨酸（GAMA）的固相萃取-高效液相色谱质谱联用（SPE-LC-MS/MS）方法，采用该方法对吸烟者和非吸烟者尿液中的AAMA和GAMA进行了测定，并将数据与采用滤嘴分析法得到的评价结果进行了相关分析。结果表明：①该方法准确灵敏，日内、日间精密度分别小于7%和11%，回收率94.9%~100.0%，AAMA和GAMA的检测限分别为0.6和0.1 ng/mL。②吸烟者尿液中AAMA和GAMA均值是非吸烟者的3倍左右。③尿液中丙烯酰胺主要代谢物AAMA的质量浓度与应用滤嘴分析法测得的烟气丙烯酰胺暴露量有一定相关性（R=0.567，P＆lt;0.03）。

In order to assess the exposure to acrylamide of a smoker during smoking accurately, an SPE-HPLC-MS/MS method was developed for determining the mercapturic acid （MA） metabolites, N-acetyl-S-（2-carbamoylethyl）-L-cysteine （AAMA） and N-acetyl-S-（2-carbamoyl-2-hychoxyethyl）-L-cysteine（GAMA）, of acrylamide in human urine.The contents of AAMA and GAMA in the urine of smokers and nonsmokers were determined, and the correlation analysis between the determined data and the acrylamide exposure evaluated by filter analysis was conducted. The results showed that： 1）The method was accurate and sensitive with the intra-day and inter-day precisions less than 7% and 11%, respectively. The recoveries ranged from 94.9%to 100.0%, and the detection limits of AAMA and GAMA were 0.6 and 0.1 ng/mL, respectively. 2） The mean value of AAMA and GAMA in smokers？？urine was three times the value in nonsmokers’. 3） The content of AAMA, the main metabolite of acrylamide, in urine had a certain correlation（R=0.567, P＆lt;0.03）with the acrylamide exposure determined by filter analysis.