弱光环境对罗勒幼苗叶片生长和叶绿素荧光特性的影响

Effects of growth characteristics and chlorophyll fluorescence characteristics in leaves of Ocimum basilicum L. seedlings under low light intensity

以花色素苷含量不同的紫罗勒和大叶罗勒为对象,分别对其进行弱光和自然光处理30d后进行生长参数、色素含量和叶绿素荧光参数的测定,结果显示:弱光导致两种罗勒幼苗叶片比叶面积、叶面积、叶宽、叶长和株高显著增加,幼苗根长,地上、地下生物量和总生物量明显降低。经过弱光处理的叶片花色苷与总叶绿素比和花色苷含量均降低。总叶绿素含量、叶绿素a含量、叶绿素b含量和叶绿素a/b比值较自然光明显升高。在弱光下,两种罗勒幼苗叶片失活PSⅡ反应中心的热耗散量子产额(ФNF)降低,而基本的荧光量子产额和热耗散的量子产额(Фf,D)升高。说明,花色素苷可能在光破坏防御上发挥了作用。

Based on Ocimum basilicum L. seedlings with different content of anthocyanins, this article studied on the growth parameters, pigment content and chlorophyll fluorescence parameters dealed with low light intensity and nature light intensity for 30 days. Finally, the specific leaf area, leaf area, blade width, leaf length, plant height of leaves of Ocimum basilicum L. seedlings increased significantly, however root length, aboveground biomass, underground biomass, total biomass of leaves decreased significantly with low light intensity. The content of anthocyanins and the ratio of anthocyanins and total chlorophyll of Ocimum basilicum L. seedlings with low light intensity was lower than the one with nature light intensity,however the content of total chlorophyll, chlorophyll a, chlorophyll b, the ratio of chlorophyll a and b of Ocimum basilicum L. seedlings with low light intensity was higher. Dealed with low light intensity, the quantum yield of thermal dissipation in nonfunctional PSⅡ （ФNF） decreased, the basic quantum yield of fluorescence and thermal dissipation（Фf,D） increased in leaves of Ocimum basilicum L. seedlings. The result showed that anthocyanins may have the effect on the defense of light damage.