中医药治疗慢性萎缩性胃炎的相关进展研究——养阴活胃合剂对慢性萎缩性胃炎模型大鼠血清炎症因子的影响

Effect of Yangyinhuowei mixture on serum inflammatory factors in rats with chronic atrophic gastritis

目的探讨养阴活胃合剂对慢性萎缩性胃炎（CAG）模型大鼠血清炎症因子含量的影响及其作用机制。方法将实验动物随机分为6组：空白对照组、模型组、阳性药物对照组及中药低剂量组、中剂量组、高剂量组，每组16只。除空白对照组常规饲养、自由进食水之外，其余5组将2 g 水杨酸钠加入100 mL 的30％乙醇溶液中，给大鼠灌胃，每日1次，每次3 mL，配合隔日喂食不禁水法建立大鼠萎缩性胃炎模型，模型复制成功后中药治疗低、中、高剂量组分别喂饲养阴活胃合剂水煎剂0．74 g·kg^－1·d^－1、1．48 g·kg^－1·d^－1和2．22 g·kg^－1·d^－1。12 w 后测定血清中 IL-6、TNF-α、IFN-β及 NOS2含量。结果养阴活胃合剂能够改善大鼠胃黏膜病理状态，使病变胃黏膜趋于正常。与空白对照组比较，各组 IL-6、TNF-α、IFN-β及 NOS2均明显升高（P ＜0．05），证明模型复制成功。与模型组相比，中药高剂量组 TNF-α、IL-6、NOS2均明显降低，差异有统计学意义（P ＜0．05），中药中剂量组 IFN-β降低，差异有统计学意义（P ＜0．05）。结论养阴活胃合剂可降低慢性萎缩性胃炎大鼠血清中炎症因子水平，而抑制炎症反应可能有助于慢性萎缩性胃炎病情的改善。

Objective The study is to investigate the effect of Yangyinhuowei mixture on the content of ser-um inflammatory factors in rat with chronic atrophic gastritis （CAG）and its treating mechanism.Methods The experimental animals were randomly divided into 6 groups：blank control group,model group,pos-itive drug group,low dose of Chinese medicine group,middle dose group and high dose group,16 rats in each group.Except that blank control group was conventionally fed in normal way and with free access to food and water,the rats in other groups were fed by 30% ethanol solution and 2 g sodium salicylate adding 100 mL,1 time a day,each time 3 mL,thus establishing chronic atrophic gastritis rat models.The rats in these models were then given the treatment of traditional Chinese medicine Yangyinhuowei mixture decoc-tion with the dosage of 0.74 g·kg^-1 ·d^-1 ,1.48 g·kg^-1 ·d^-1 and 2.22 g·kg^-1 ·d^-1 .The serum levels of IL-6, TNF-alpha, IFN-beta and NOS2 levels were determined within 12 weeks.Results Yangyinhuowei mixture decoction can improve the pathological state of rat gastric mucosa,helping it recover to normal.Compared with the blank control group,IL-6,TNF-alpha,IFN-beta and NOS2 in other groups were significantly increased （P 〈0.05）.It proved that the model could be replicated successfully. Compared with the model group,TNF-alpha,IL-6,NOS2 in high dose of Chinese medicine group decreased,and the difference was statistically significant （P 〈0.05）.IFN-beta decreased in Chinese medicine dose group,and the difference was statistically significant （P 〈 0.05 ）.Conclusion Yangyinhuowei mixture decoction can reduce the levels of inflammatory cytokines in serum in rats with chronic atrophic gastritis,and inhibition of inflammation may contribute to the recovery of chronic atrophic gastritis.