三种情绪稳定剂抑制哇巴因上调星形胶质细胞ERK_(1/2)磷酸化水平

Three Mood Stabilizers for Inhibition of Ouabain-induced ERK_(1/2) Phosphorylation in Astrocytes

目的探讨锂盐等3种情绪稳定剂对哇巴因上调星形胶质细胞ERK1/2磷酸化水平的作用。方法将培养星形胶质细胞随机分为生理盐水对照组、哇巴因组、情绪稳定剂组(碳酸锂、卡马西平和丙戊酸钠)和哇巴因+情绪稳定剂组。采用Western印迹法半定量检测各组ERK1/2磷酸化水平。结果哇巴因组ERK1/2磷酸化水平升高,与对照组和情绪稳定剂组相比,差异有统计学意义(P<0.05),而哇巴因+情绪稳定剂组与对照组和抑制剂组相比,差异无统计学意义。结论 3种情绪稳定剂能抑制哇巴因上调星形胶质细胞ERK1/2磷酸化水平。

Objective To investigate the effets of three mood stabilizers on ouabain-induced ERK1/2phosphorylation in astrocytes. Methods Astrecytes were treated with different agents and divided into different groups accordingly, namely, the control group with saline, the group with oua- bain, the group with mood stabilizers （lithium carbonate, carbamazepine, sodium valproate） and the group with ouabain ＋ mood stabilizers. The phosphorylafion of ERK1/2 in each group was analyzed by Western blot. Results Compared with saline and mood stabilizer groups, the pliosphorylation of ERK1/2 was increased in the ouabain group, with statistical significance （P 〈 0.05 ）. There was no significant difference in ERK1/2 phosphoryla- tion between the group with ouabain ＋ mood stabilizers and the control or mood stabilizer group. Conclusion The three kinds of mood stabilizers can inhibit ouabain-induced ERK1/2 phosphorylation in astrocytes.