血清与尿液中RANKL检测在骨质疏松诊断中的意义

Clinical Significance of Serum and Urine RANKL Test for Diagnosis of Osteoporosis

目的探讨血清与尿液中核因子kappa B(NF-κB)受体活化因子配体(RANKL)检测在骨质疏松诊断中的意义。方法选取53例骨质疏松患者(实验组)和45例健康者(正常对照组)为研究对象。用ELISA方法测定血清与尿液中RANKL的表达水平并进行比较。结果实验组血清与尿液中RANKL的水平明显高于正常对照组,差异有统计学意义(P<0.01)。血清与尿液中RANKL的ROC曲线下面积分别为0.898和0.734。联合检测血清与尿液RANKL和Ca2+水平对骨质疏松诊断的灵敏度为89.5%,特异度为86.1%。结论 RANKL可能与骨质疏松的发生、发展有关,血清与尿液中RANKL的检测可用于骨质疏松的辅助诊断。

Objective To investigate the significance of test of receptor activator of nuclear factorkappa B （ NF - κB ） ligand （RANKL） in serum and urine for the diagnosis of osteoporosis. Methods A total of 53 patients with osteoporosis （the experimental group） and 45 healthy controls （the normal control group） were recruited in this study. The expression levels of RANKL in serum and urine was measured and compared by enzyme- linked immunosorbent assay. Results The serum and urine levels of RANKL in the experimental group were significantly higher than those in the normal control group （P 〈 0.01 ）. The areas under receiver operating characteristic （ROC） curve of serum and urine RANKL were 0.898 and 0.734, respectively. The combined detection of serum and urine RANKL and Ca2＋ reached a high sensitivity of 89.5% and a specificity of 86.1% for diagno- sis of osteoporosis. Conclusion RANKL may be closely associated with the progression of osteoporosis. Serum and urine RANKL test may be help- full in the diagnosis of osteoporosis.