DBC2在胃癌中的甲基化

Study on Methylation of DBC2 in Gastric Cancer

目的检测DBC2在胃癌组织中的表达水平及其基因启动子区的甲基化状态,从而探讨二者的相关性。方法选取92例胃癌组织及其相应的癌旁组织为研究对象,通过RT-PCR方法检测DBC2 m RNA表达水平,并利用MS-PCR方法检测DBC2启动子区的甲基化状态,同时分析胃癌组织中DBC2启动子区甲基化水平与临床病理因素的相关性。结果与癌旁组织相比,胃癌组织中DBC2的表达水平降低,同时伴随其启动子区高甲基化。其甲基化水平与肿瘤浸润深度、分化程度及TNM分期密切相关(P<0.05),随着肿瘤浸润深度的增加、分化程度的降低以及TNM分期的提高,胃癌组织中DBC2启动子区具有更明显的甲基化水平。结论胃癌中DBC2启动子区高甲基化可能是导致其表达水平降低的一个重要原因。

Objective To detect the expression of DBC2 and the methylation status of its gene promoter region in gastric cancer, so as to analyze their correlation. Methods Gastric cancer tissues and adjacent normal tissues were collected from 92 cases as research subjects. The DBC2 mRNA expression and the methylation status of DBC2 gene promoter region in gastric cancer were detected by using RT-PCR and MS-PCR respectively. In addition, the correlation of DBC2 gene methylation status with elinicopathological parameters was analyzed. Results In comparison with adjacent normal tissues, gastric cancer tissues displayed a low expression level of DBC2 mRNA and a high methylation level of gene promoter region. Besides, the methylation level was significantly correlated with tumor invasion depth, differentiation and TNM staging （ P 〈 0.05 ）. With deep invasion, poor differentiation or higher TNM staging, methylation level of gene promoter region was more significant in gastric cancer tissues. Conclusion High methylation of DBC2 gene promoter region may be a significant contributing factor leading to low DBC2 expression.