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五味子水溶性蛋白质的提取工艺优化 被引量:6

Optimization of Extraction Process of Water-soluble Protein from Schisandrae Chinensis Fructus
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摘要 目的:探讨碱提酸沉法提取五味子水溶性蛋白质的工艺条件。方法:以蛋白质提取率为评价指标,采用单因素试验和正交试验考察料液比、提取温度、提取时间及提取液p H对五味子水溶性蛋白质提取工艺的影响,分析水溶性蛋白质的等电点及分子组成。采用考马斯亮蓝Bradfrod法测定提取液中蛋白质含量。结果:五味子蛋白质的等电点3.4。最佳提取条件为料液比1∶35,提取温度35℃,提取时间2 h,提取液p H 9.5,水溶性蛋白质提取率43.62%。SDS-PAGE电泳分析表明五味子水溶性蛋白质主要含有50,40,38,21 k Da的亚基和少量18,16 k Da的亚基。结论:优选的五味子水溶性蛋白质提取工艺稳定可行,为该成分的功能性保健食品开发提供参考。 Objective:To optimize alkali extraction and acid precipitation process of water-soluble protein from Schisandrae Chinensis Fructus.Method:With extraction rate of protein as index,single factor tests and orthogonal design were adopted to optimize extraction process of water-soluble protein by taking solid-liquid ratio,pH of extract,extraction temperature and time as factors,isoelectric point and molecular components of water-soluble protein were analyzed.Coomassie brilliant blue Bradfrod was employed to determine protein content in extract.Result:Isoelectric point of water-soluble protein was 3.4.Optimum extraction conditions was as following:solid-liquid ratio of 1 ∶ 35,pH of extract 9.5,extraction temperature at 35 ℃,extraction time of 2 h.Extraction rate of water-soluble protein was up to 43.62%.SDS-PAGE analysis showed that majority subunits of water-soluble protein were 50,40,38,21 kDa,and a small amount of 18,16 kDa.Conclusion:This optimized extraction process is stable and feasible,this study provides a reference for development of functional health food by water-soluble protein from Schisandrae Chinensis Fructus.
机构地区 长春中医药大学
出处 《中国实验方剂学杂志》 CAS CSCD 北大核心 2015年第2期16-19,共4页 Chinese Journal of Experimental Traditional Medical Formulae
基金 吉林省重点科技攻关项目(20140204070YY)
关键词 五味子 水溶性蛋白质 等电点 电泳 碱提酸沉法 Schisandrae Chinensis Fructus water-soluble protein isoelectric point electrophoresis alkali extraction and acid precipitation method
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  • 1Kang S,Lee K P,Park S J,et al.Identification of a novel anti-inflammatory compound,α-cubebenoate from Schisandra chinensis[J].J Ethnopharmacol,2014,153(1):242-249.
  • 2Qu H M,Liu S J,Zhang C Y.Antitumor and antiangiogenic activity of Schisandra chinensis polysaccharide in a renal cell carcinoma model[J].Int J Biol Macromol,2014,66(1):52-56.
  • 3Zhao T,Mao G,Mao R,et al.Antitumor and immunomodulatory activity of a water-soluble low molecular weight polysaccharide from Schisandra chinensis (Turcz.) Baill[J].Food Chem Toxicol,2013,55 (1):609-616.
  • 4Zhang M,Liu M,Xiong M,et al.Schisandra chinensis fruit extract attenuates albuminuria and protects podocyte integrity in a mouse model of streptozotocin-induced diabetic nephropathy[J].J Ethnopharmacol,2012,141(1):111-118.
  • 5王丽薇,周长新,Bernd Schneider,赵昱.北五味子化学成分研究[J].中国现代应用药学,2006,23(5):363-365. 被引量:21
  • 6戴好富,周俊,彭再刚,谭宁华.北五味子的水溶性化学成分[J].天然产物研究与开发,2001,13(1):24-26. 被引量:57
  • 7戴好富,谭宁华,周俊,易元芬.北五味子挥发性化学成分研究[J].中草药,2005,36(9):1309-1310. 被引量:26
  • 8林伟卓,陈玉海.北五味子全氮及氨基酸含量测定[J].特产研究,1998,20(1):35-36. 被引量:9
  • 9万志强,严铭铭,展月,邵帅,于雷,于红威,赵大庆.五味子蛋白的提取纯化及含量测定[J].中国实验方剂学杂志,2011,17(17):119-122. 被引量:10
  • 10Bradford M M.A rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding[J].Anal Biochem,1976,72(1):248-254.

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