摘要
目的:采用高效液相色谱法建立白芷中香豆素类成分的指纹图谱,并同时测定欧前胡素和异欧前胡素的含量。方法:采用Platisil DOS C18色谱柱(4.6 mm×250 mm,5μm),乙腈-水为流动相进行梯度洗脱,检测波长254 nm。共收集了10批白芷进行测定,运用国家药典委员会"中药色谱指纹图谱相似度评价软件"2004A版进行评价,并对2种成分进行含量测定。结果:建立了白芷香豆素类成分HPLC指纹图谱,标定了10个共有色谱峰。含量测定条件通过方法学验证,平均加样回收率分别为102.52%,98.23%。结论:该法所建立的白芷香豆素类成分指纹图谱特征性强、方法简便,结合欧前胡素和异欧前胡素含量测定可更好地控制其质量,对白芷的鉴定及质量控制具有指导意义和参考价值。
Objective:A method of HPLC-DAD was developed to evaluate the quality of Angelicae Dahuricae Radix through multi-target ingredient determination and finger printing analysis technologies.Method:The optimal conditions of separation and detection were achieved on a Platisil DOS C18 (4.6 mm ×250 mm,5 μm) with linear gradient elution of acetonitrile-water.The detection wavelength was 254 nm.Collect 10 batches from different origins in China to assess the similarities according to similarity evaluation for ‘ chromatographic fingerprint of traditional Chinese medicine’ (2004A).Result:In quantitative analysis,the two components showed good regression with linear ranges,and their recoveries were 102.52% and 98.23%.Conclusion:This study indicated that the combination of quantitative and chromatographic fingerprint analysis can be readily utilized as a quality control method for Angelicae Dahuricae Radix.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第2期36-39,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十一五"科技支撑计划项目(2008BAI51B03)
国家自然科学基金青年基金项目(81202793)
中国中医科学院基本业务费自助选题项目(Z02063)
中央级公益性科研院所基本科研业务费专项(ZZ070819)
关键词
白芷
指纹图谱
欧前胡素
异欧前胡素
Angelicae Dahuricae Radix
finger printing
imperatorin
iso imperatorin