蒙古族药文冠木超微粉与普通粉中表儿茶素的溶出度比较

Comparison of Exoteric Dissolution of L-epicatechin from the Ultra-micro and Traditional Powder of Xanthoceras sorbifolia

目的:对蒙古族药文冠木普通粉与超微粉中主要有效成分表儿茶素的体外溶出进行考察。方法:采用HPLC测定表儿茶素含量。色谱条件为Agilengt Eclipse-C18色谱柱(4.6 mm×250 mm,5μm),检测波长280 nm,流动相乙腈-0.04%磷酸水溶液(15∶85),流速1.0 m L·min-1,进样量20μL。测定文冠木普通粉与超微粉的溶出量和溶出速率,并对体外溶出行为进行溶出动力学分析。结果:普通粉与超微粉中表儿茶素的溶出量分别为5.868,8.440 mg·g-1,二者中表儿茶素的体外溶出曲线均符合Weibull分布。普通粉中表儿茶素的溶出参数T50,Td,T70,T80,T90分别为0.476,1.952,2.939,5.488,9.961 min,超微粉则依次为0.180,0.348,0.469,0.768,1.249 min。超微粉中表儿茶素的溶出速率明显高于普通粉(P<0.01),单位时间内(1 h)体外溶出量较普通粉提高约44%。结论:超微粉碎能明显促进文冠木中表儿茶素的体外溶出速率及溶出量,说明超微粉碎技术应用于蒙古族药文冠木是切实可行的。

Objective：To investigate the dissolution of L-epicatechin from the ultra-micro and traditional powder of Xanthoceras sorbifolia.Method：HPLC technique was used to detect the content of L-epicatechin.Chromatographic conditions were as follows：Agilent Eclipse-C18 column （4.6 mm × 250 mm,5 μm）,detection wavelength 280 nm,mobile phase of acetonitrile-0.04% H3PO4 （15：85）,flow rate 1.0 mL· min^-1,injection volume 20 μL.Dissolution amount and the dissolution rate of L-epicatechin from the ultra-micro and traditional powder of X.sorbifolia were determined,and exoteric dissolution behavior was analyzed.Result：Dissolution amount of L-epicatechin from ultra-micro and traditional powder were 5.868 mg ·g^-1and 8.440 mg · g,respectively.Exoteric dissolution curves were in line with Weibull distribution.T50,Td,T70,T80,Tg0 of traditional powder were 0.476,1.952,2.939,5.488,9.961 min,respectively,but these of ultra-micro powder were 0.180,0.348,0.469,0.768,1.249 min.The dissolution rate of ultra-micro powder of X.sorbifolia was increased obviously （P 〈 0.01）.Per unit of time （1 h） dissolution amount increased by about 44% compared with traditional powder.Conclusion：Superfine grinding can promote the dissolution of L-epicatechin in X.sorbifolia,and application of superfine grinding is feasible and necessary.