窄叶芍药的化学成分及其抗氧化活性

Chemical Constituents and Antioxidant Evaluation of Paeonia anomala

目的：研究窄叶芍药的化学成分,并对分离得到的化合物进行抗氧化活性评价.方法：采用反复硅胶柱色谱法和Sephadex LH-20柱色谱法等进行分离纯化,并通过理化常数测定和光谱分析鉴定其化学结构;并采用1,1-二苯基-2-硝基苯肼自由基清除法（DPPH）和2,2-联氮基-双-（3-乙基苯并噻唑啉磺酸-6）二铵盐自由基清除法（ABTS）对化合物的体外活性进行评价.结果：从窄叶芍药中分离得到5个化合物,并鉴定为没食子酸乙酯（1）,没食子酸甲酯（2）,齐墩果酸（3）,丁香酸（4）,β-谷甾醇（5）;对化合物1～4进行抗氧化活性测试,结果表明：阳性药（维生素C,VC）及化合物1和2对DPPH自由基的半数清除率（IC50）分别达到（35.13±0.28）,（44.64±0.37） μmol·L^-1,均低于阳性对照抗坏血酸IC50（60.17±0.98）μmol·L^-1;阳性药及化合物1和2对ABTS自由基的IC50分别达到（25.13-0.68）,（35.76±1.48） μmol·L^-1,均低于阳性对照抗坏血酸IC50（38.55 ±0.78） μmol·L^-1.结论：化合物3,4首次从该植物中分离得到,并且化合物1和化合物2具有很强的抗氧化活性,其余化合物的抗氧化活性较弱.

Objective：To study the chemical constituents from Paeonia anomala and to evaluate their antioxidant activities.Method：The compounds were isolated by chromatography on silicagel,Sephadex LH-20 column and identified on the basis of physicochemical constants and spectral analysis.Their antioxidant activities were evaluated by 1,1-diphenyl-2-pieryl-2-picrylhydrazyl （DPPH） and 2,2-amino-di （3-ethyl-benzothiazoline sulphonic acid-6） ammonium salt （ABTS） radical scavenging methods.Result：Five compounds were isolated and their structures were identified as ethyl gallate （1）,methyl gallate （2）,oleanic acid （3）,syringate （4） and β-sitosterol （5）.The results showed that the DPPH radical scavenging activity of compounds 1 and 2 were lower than the positive control of Vitamin C with IC50values （35.13 ± 0.28）,（44.64 ± 0.37） μmol ·L^-1 Their ABTS radical scavenging activity were lower than Vitamin C with IC50values of （25.13 ±0.68）,（35.76 ± 1.48） μmol · L^-1,respectively.Conclusion：Compounds 3,4 are isolated from this plant for the first time.Compounds 1,2 have powerful antioxidant activies,and the others have little antioxidant activies.