摘要
目的:建立HPLC同时测定健脾舒胃凝胶中甘草苷、柚皮苷、橙皮苷、新橙皮苷、甘草酸铵5种成分含量控制方法.方法:采用Agilent ZORBAX Eclispse SB-C18色谱柱(4.6 mm ×150 mm,5μm),流动相乙腈-0.05%磷酸水梯度洗脱,流速1.0 mL·min^-1,检测波长237 nm(甘草苷、甘草酸铵),283 nm(柚皮苷、橙皮苷、新橙皮苷).结果:根据回归方程,甘草苷、柚皮苷、橙皮苷、新橙皮苷、甘草酸铵分别在0.1283~0.6416,1.1052~5.526,0.2252~1.1260,1.092~5.460,0.3149~1.5744μg进样量与峰面积之间线性关系良好;5个成分的平均回收率分别为101.47%,99.22%,100.02%,101.64%,97.99%(RSD<3%).结论:该方法简便、准确可靠,重复性好,专属性好,分离效果好,适用于健脾舒胃凝胶的质量控制.
Objective:To develop a method for determining liquiriti,naringin,hesperidin,neohesperidin and glycyrrhizic acid ammonium salt in Jianpi Shuwei gel.Method:The mobile phase consisted of acetonitrile-0.05% phosphoric acid with gradient elution at the flow rate of 1.0 mL · min^-1 The detection wavelength was 237 nm (liquiriti,glycyrrhizic acid ammonium salt),283 nm (naringin,hesperidin,neohesperidin).Result:The liquiriti,naringin,hesperidin,neohesperidin and glycyrrhizic acid ammonium salt all had good linear relationship in the ranges of 0.1283-0.641 6,1.105 2-5.526,0.225 2-1.126 0,1.092-5.460,0.3149-1.5744 μg.The average recoveries were 101.47%,99.22%,100.02%,101.64% and 98.11% (RSD 〈3%),respectively.Conclusion:The method is simple,accurate,reproducible and nice to the separation,can be used for the quality evaluation of Jianpi Shuwei gel.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第2期77-79,共3页
Chinese Journal of Experimental Traditional Medical Formulae