迈康片对高脂血症大鼠血管保护的作用机制研究

Protective Effect of Maikang Tablets on Blood Vessel in Experimental Hyperlipidemic Rats

目的:研究迈康片对高脂血症大鼠血管的保护及作用机制。方法:根据血清总胆固醇(TC)水平将实验大鼠随机分为基础饲料正常组、高脂饲料模型组和迈康片低(0.47 g·kg-1)、中(0.94 g·kg-1)、高(1.88 g·kg-1)3个剂量组。高脂饲料模型组和迈康片各剂量组每日以高脂饲料喂养,正常组以基础饲料喂养。各组分别ig各剂量药物,持续30 d,测定各组大鼠血清中血脂水平及丙二醛(MDA)、内皮素-1(ET-1)、降钙素基因相关肽(CGRP)、前列环素(PGI2)、内皮源性超极化因子(EDHF)、一氧化氮(NO)的含量及超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)的活性。结果:与正常组比较,模型组的体质量无太大变化,模型组高脂血症大鼠TC,甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),MDA,ET-1,CGRP,PGI2,EDHF,NO含量明显升高(P<0.05,P<0.01),NOS活性显著升高(P<0.01),高密度脂蛋白胆固醇(HDL-C)含量、SOD活性显著降低(P<0.01);与模型组比较,迈康片对受试动物体质量无影响,而迈康片均能使高脂血症大鼠TC,TG,LDL-C,MDA,ET-1,CGRP,PGI2,EDHF,NO含量明显降低(P<0.05,P<0.01),NOS活性显著降低(P<0.05,P<0.01),HDL-C含量,SOD活性显著升高(P<0.05,P<0.01)。结论:迈康片对高脂血症模型大鼠血管具有显著的保护修复作用。

Objective：To determine the protective effects of Maikang tablets on blood vessel in the experimental hyperlipidemic rats.Method：The rats were randomly divided into 5 groups according to their baseline total cholesterol （TC） level,including the control group,the hyperlipidemia model group,the lowmiddle-and high-dose Maikang tablets groups （0.47,0.94,1.88 g ·kg^-1）.The rats in the control group were fed with basal diet,the rats in the hyperlipidemia model and Maikang tablets groups were fed with high-fat diet,and the rats in Maikang tablets groups added Maikang tablets.All rats were intragastrically administrated of the corresponding medicines for 30 days.The level of blood lipid and the contents of malonaldehyde （MDA）,nitric oxide （NO）,endothelin （ET）-1,calcitonin generelated peptide （CGRP）,prostacyclin （PGI2）,endotheliumderived hyperpolarizing factor （EDHF） were determined and the activity of nitric oxide synthase （NOS）,superoxide dismutase （SOD） of the rats were tested.Result：Compared with the normal group,the body weight of rats in the model group had little change.The contents of hyperlipidemia TC,triglyceride （TG）,low density lipoprotein cholesterol （LDL-C）,MDA,ET-1,CGRP,PGI2,EDHF,NO increased obviously （P 〈 0.05,P 〈 0.01）,the activity of NOS increased significantly （P 〈0.01）,the content of high density lipoprotein cholesterol （HDL-C） and the activity of SOD decreased significantly （P 〈 0.01） in the hyperlipidemia modle group.Maikang tablets also had no effect on body weight,while it could improve the above indicators significantly （P 〈 0.05,P 〈 0.01）.Conclusion：Maikang tablets have good effects on blood vessel protection and vascular repair function in rats with hyperlipidemia.