桑叶生物碱、黄酮及多糖对HepG2细胞胰岛素抵抗的改善作用

Influence of Different Effective Extracts of Mori Folium on Hep G2 Cells

目的:探讨桑叶生物碱、黄酮及多糖对Hep G2细胞胰岛素抵抗的改善及其作用机制。方法:采用高糖高胰岛素培养基诱导Hep G2细胞形成红外(IR)模型,葡萄糖氧化酶法检测正常组、模型组、罗格列酮组、生物碱组、黄酮组及多糖组的细胞葡萄糖消耗情况,并用RT-q PCR法检测C-Jun氨基端激酶(JNK)通路相关基因的表达,观察桑叶生物碱、黄酮及多糖改善Hep G2细胞胰岛素抵抗及其作用机制。结果:高糖及高胰岛素培养基培养Hep G2细胞,使细胞葡萄糖消耗率明显下降,造成胰岛素抵抗的细胞模型,给予桑叶生物碱、黄酮及多糖干预之后,细胞葡萄糖消耗率明显上升,同时下调JNK和上调IRS-1,AKT mRNA的表达。结论:桑叶生物碱、黄酮及多糖均可增加Hep G2细胞的葡萄糖消耗量,其改善胰岛素抵抗作用可能与调节JNK信号通路有关。

Objective：To investigate the protective effect and its mechanism of different effective extracts of Mori Folium on the infrared （IR） model of HepG2 cells.Method：High glucose and insulin in HepG2 cells to form a cell model of insulin resistance,glucose oxidase detect the expression of glucose consumption,and using the real-time fluorescent quantitative polymerase chain reaction （PCR） technique to observe the expression level of C-Jun amino termind kinose （JNK） pathway related genes in different effective extracts of Mori Folium on the IR model of HepG2 cells.Result：High sugar and high insulin culture environment capable of inducing cells to produce insulin resistance.All of the groups can increase the glucose consumption （P ＜ 0.01） and has the influence on the expression of JNK related genes.Conclusion：The alkaloids,flavonoids and polysaccharides of mulberry leves can increase the glucose consumption on the HepG2 cells,which may be related to regulating JNK signaling pathways.