乌腺金丝桃愈伤组织中总黄酮及金丝桃素含量测定

Determination the Content of Total Flavonoids and Hypericin in Callus from Hypericum attenuatum Choisy

以乌腺金丝桃新鲜叶片为材料,建立稳定的乌腺金丝桃愈伤组织的培养体系,进一步对愈伤组织总黄酮与金丝桃素的含量进行测定。以MS为基础培养基,考察不同激素组合对愈伤组织诱导的影响。结果表明,乌腺金丝桃诱导愈伤组织的最佳培养基为MS+2,4-D 4.0 mg/L+6-BA 0.2 mg/L;采用超声波法提取乌腺金丝桃愈伤组织中的总黄酮和金丝桃素,分别采用紫外分光光度法和HPLC法测定乌腺金丝桃愈伤组织中总黄酮及金丝桃素的含量,结果表明,干燥乌腺金丝桃愈伤组织中总黄酮含量为27.99 mg/g,金丝桃素的含量为0.515 mg/g。

With fresh leaves as test material, this study established a relatively stable system of callus culture forHypericum attenuatum Choisy, and determined the content of total flavonoids and hypericin in callus. With MS as the basic culture medium, the effects of different hormone combinations were investigated on callus induction. The results showed that the optimum culture media for callus induction was MS ＋ 2,4-D 4.0 mg/L ＋ 6-BA 0.2 mg/L. The total flavonoids and hypericin of callus were extracted using ultrasonic method, respectively, using UV spectrophotometry and HPLC method for determination total flavonoids content and hypericin content in callus. The results showed that total flavonoids was 27.99 mg/g, and hypericin content was 0.515 mg/g in dry callus.