生川乌与生半夏不同比例配伍对大鼠肾脏毒性的影响

Impact on Renal Toxicity with the Compatibility of Different Proportions of Raw Chuanwu( Aconitum carmichaelii) and Raw Banxia( Pinellia ternata)

目的探讨生川乌与生半夏的配伍比例与大鼠肾脏毒性的关系。方法按1∶0、4∶1、2∶1、1∶1比例制作生川乌、生半夏冻干粉。将50只SD大鼠随机分为正常对照组、生川乌组、川乌4半夏1组、川乌2半夏1组、川乌1半夏1组,每组10只。保证各给药组大鼠生川乌给药剂量均为0.8 g/kg的前提下,按比例灌胃给予各给药组冻干粉溶液4 ml/kg,正常对照组给予等体积生理盐水。灌胃4周末次给药后24 h及停药2周时测定大鼠尿液中微量白蛋白(m Alb)、转铁蛋白(TRF)和血清中尿素氮(BUN)、肌酐(SCr)、β2微球蛋白(β2-MG)含量;计算肾脏指数并进行形态学观察。结果给药4周后,除川乌1半夏1组血清BUN外,各给药组大鼠m Alb、TRF、BUN、SCr、β2-MG含量明显高于正常对照组(P<0.05或P<0.01);生川乌组、川乌4半夏1组、川乌2半夏1组大鼠肾脏指数明显高于正常对照组(P<0.05或P<0.01);生川乌组上述检测指标均明显高于其余给药组(P<0.05或P<0.01),川乌2半夏1组与川乌1半夏1组比较差异无统计学意义(P>0.05)。停药2周后,各组肾脏指数、m Alb、TRF、BUN、SCr、β2-MG含量差异无统计学意义(P>0.05)。结论生川乌对大鼠肾脏具有明显毒性,生川乌配伍不同比例生半夏后其肾脏毒性降低,且在一定比例范围内毒性随生半夏配伍比例的升高而降低。

Objective To discuss the impact on renal toxicity with the compatibility of raw Chuanwu（ Aconitum carmichaelii,川乌） and raw Banxia（ Pinellia ternata,半夏） and its relation with different proportions. Methods Raw Chuanwu（ Aconitum carmichaelii,川乌） and raw Banxia（ Pinellia ternata,半夏） mixed lyophilized powders with the proportion of 1 ∶ 0,4 ∶ 1,2 ∶ 1,and 1 ∶ 1 were made. Fifty SD rats were randomly divided into control group,Chuanwu group,Chuanwu-4 Banxia-1 group,Chuanwu-2 Banxia-1 group,and Chuanwu-1 Banxia-1 group,with 10 in each. In addition to being given the same amount of Chuanwu（ 0. 8 g / kg）,each group was administered with the solution（ 4 ml / kg） of mixed lyophilized powder in the correspondence set proportion,and the control group received an equal volume of normal saline. Levels of microalbumin（ m Alb） and transferring（ TRF） in urine and blood urea nitrogen（ BUN）,serum creatinine（ SCr）,and β2-microglobulin（ β2-MG） were tested 24 h after last administration of 4 weeks gavage and 2 weeks after withdrawal. Renal index was calculated and morphology was observed. Results After 4 weeks of administration,levels of m Alb,TRF,BUN,SCr,and β2-MG in each treatment group were significantly higher than those in the control group（ P〈0. 05 or P〈0. 01）,except for the serum level of BUN in the Chuanwu-1 Banxia-1 group. The renal index in Chuanwu group,Chuanwu-4 Banxia-1 group,and Chuanwu-2Banxia-1 group were significantly higher than that the control group（ P〈0. 05 or P〈0. 01）. The above indicators in Chuanwu group were significantly higher than those in other treatment groups（ P〈0. 05 or P〈0. 01）,and there were no significant differences in indicators between the Chuanwu-2 Banxia-1 group and the Chuanwu-1 Banxia-1 group（ P〉0. 05）. Two weeks after drug withdrawal,there were no statistical differences in renal index,levels of m Alb,TRF,BUN,SCr,and β2-MG among groups（ P〉0. 05）. Conclusion Raw Chuanwu（ Aconitum carmichaelii,川乌） has obvious renal toxicity,but with the compatibility of different proportions of raw Banxia（ Pinellia ternata,半夏）,the toxicity is alleviated. Also,within a certain range,renal toxicity decreased with the proportion of raw Banxia（ Pinellia ternata,半夏） escalating.