HPLC法测定多叶越南槐中红车轴草苷及高丽槐素的含量

HPLC method for simultaneous determination of trifolirhizin and maackiain in Sophora tonkinensis Gagnep. var.polyphylla S. Z. Huang et Z. C. Zhou

目的：建立测定多叶越南槐药材中红车轴草苷及高丽槐素2个黄酮类成分含量的高效液相色谱法。方法：采用Agilent Eclipse XDB-C18色谱柱（4.6 mm×250 mm,5μm）,柱温25℃,以乙腈-0.1%乙酸水溶液为流动相,梯度洗脱,流速1.0 m L·min^-1,检测波长310 nm,进样量10μL,外标法定量。结果：红车轴草苷进样量在0.025 1-2.51μg范围内与峰面积呈良好的线性关系（r=0.999 0）,平均回收率为97.0%（RSD=1.5%）;高丽槐素进样量在0.025 2-2.52μg范围内与峰面积呈良好的线性关系（r=0.999 0）,平均回收率为101.3%（RSD=2.2%）。结论：建立的含量测定方法符合方法验证要求。

Objective： To establish an HPLC method to determine the content of two flavonoids（ trifolirhizin and maackiain） in Sophora tonkinensis Gagnep. var. polyphylla S. Z. Huang et Z. C. Zhou. Methods： An Agilent Eclipse XDB-C18column（ 4. 6 mm × 250 mm,5 μm） was adopted,and the mobile phase consisted of acetonitrile and0. 1% aqueous acetic acid with a gradient elution. The flow rate was 1. 0 m L·min^-1,the column temperature was25 ℃,the detection wavelength was 310 nm and the injection volume was 10 μL,the sample was quantified with external standard. Results： The calibration curve of trifolirhizin was in good linearity at 0. 025 1-2. 51 μg（ r = 0. 999 0）and the average recovery was 97. 0% with RSD of 1. 5%. The calibration curve of maackiain was in good linearity at0. 025 2-2. 52 μg（ r = 0. 999 0） and the average recovery was 101. 3% with RSD of 2. 2%. Conclusion： The method established in this paper conforms to the requirements of the methodology validation.