EPA对大鼠学习记忆、血脂与抗氧化功能的影响

Effects of eicosapentenoic acid on learning and memory,serum lipid and antioxidant capacity of rats

研究二十碳五烯酸(EPA)对大鼠空间学习记忆能力、抗氧化功能以及血脂的影响。将24只初断乳雄性SD大鼠随机分成阴性对照组、EPA低剂量组(150 mg/(kg·d))、中剂量组(300mg/(kg·d))、高剂量组(600 mg/(kg·d))。连续饲养30 d,进行Morris水迷宫实验测试,并同步测定脑组织乙酰胆碱酯酶、一氧化氮合酶、超氧化物歧化酶和丙二醛的含量以及血清总胆固醇、高密度脂蛋白胆固醇、甘油三酯的含量。结果显示:与阴性对照组相比,EPA低剂量组丙二醛含量显著降低;EPA高剂量组逃避潜伏期、丙二醛含量显著增高;EPA中剂量组超氧化物歧化酶活力增高、丙二醛含量显著降低。研究表明EPA不能提高大鼠的空间学习记忆能力,若剂量过大还会降低大鼠的空间学习记忆功能,但一定剂量的EPA能够改善大鼠的血脂水平与抗氧化功能。

The effects of eicosapentenoic acid （ EPA） on spatial learning and memory,antioxidant capacity and serum lipid of rats were investigated. 24 weaning male SD rats were randomly divided into negative control group,low dosage EPA group（150 mg/（ kg＆#183;d） ） , mid dosage EPA group （300 mg/（ kg＆#183; d） ） and high dosage EPA group（600 mg/（ kg＆#183; d） ） . After feeding for 30 d, the Morris water maze test on the rats was conducted,and the contents of serum total cholesterol, high density lipoprotein cholesterol and triglyceride and the contents of acetylcholinesterase, nitricoxide synthase,superoxide dismutase and malondialdehyde in brain were determined. The results showed that comparing with the negative control group, the content of malondialdehyde of rats in low dosage EPA group decreased significantly; the es-cape latency and content of malondialdehyde of rats in high dosage EPA group remarkably increased;the activity of superoxide dismutase of rats improved while the content of malondialdehyde obviously declined in mid dosage EPA group. In conclusion,EPA could not improve the function of spatial learning and mem-ory of rats, and high dosage EPA could reduce the function of spatial learning and memory of rats, while certain dosage of EPA could improve the level of serum lipid and antioxidant capacity of rats.