摘要
为研究布鲁氏菌LPS对巨噬细胞中NLRP3炎症小体的影响,本试验提取布鲁氏菌2308、RB51和△WbkA的LPS,以不同浓度与小鼠巨噬细胞相互作用,荧光定量PCR检测其对NLRP3、ASC、Caspase1、IL1-β、IL18转录水平的影响。结果显示RB51LPS和△WbkA LPS上调NLRP3炎症小体相关基因的转录水平,且呈浓度依赖性,而浓度对2308LPS调节NLRP3炎症小体相关基因转录的作用不大;且同一浓度下,RB51LPS和2308LPS比△WbkA LPS更好的调节Caspase1、IL1-β、IL18的转录水平。
In order to find out the impact of Brucella LPS on macrophages NLRP3-inflammasome,the mouse macrophages were infected by the different concentrations of LPS which extracted from Brucella2308,RB51and△WbkA to explore the changes of NLRP3-inflammasome related genes,ASC,caspase1,IL1-β,IL18 at transcription levels using Real-time PCR.The results showed RB51 LPS and △WbkA LPS regulating NLRP3-inflammasome related genes transcription relied on concentrations,and concentrations of 2308 LPS had little effect on NLRP3-inflammasome related genes transcription;And under the same concentration,RB51 LPS and 2308 LPS better regulated Caspase1,IL1-β,IL18 transcriptions than △WbkA LPS.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第1期9-16,共8页
China Animal Husbandry & Veterinary Medicine
基金
国家重点基础研究发展计划(973计划)项目(2010CB530203)
