猪源多杀性巴氏杆菌荚膜分型及外膜蛋白H基因序列分析

Capsular Typing and Sequences Analysis of the ompH Gene of Pasteurella multocidafrom Swine

为了解国内猪源多杀性巴氏杆菌外膜蛋白H基因的变异情况及与荚膜型之间的相关性,本试验采用PCR方法对44株猪源多杀性巴氏杆菌进行荚膜分型和ompH基因的扩增测序。结果显示,44株菌株中22株为荚膜A型,17株为荚膜B型,5株为荚膜D型;44株菌株的ompH基因开放阅读框在1 002~1 056bp之间;SignaIP 4.1预测结果显示,信号肽为N端20个氨基酸残基;ProtParam分析结果显示,成熟蛋白氨基酸残基数量在313~331aa之间,推测的分子质量在33.83~36.46ku之间。序列分析结果显示,44株菌株核苷酸同源性为86.2%~100.0%,氨基酸同源性为86.0%~100.0%;ompH基因核苷酸序列遗传进化树结果显示,荚膜A型、B型和D型菌株分别在不同的分支。试验结果表明,猪源多杀性巴氏杆菌ompH基因在不同血清型之间具有较高的同源性,与荚膜型之间存在相关性。

In order to understand the variation of outer membrane protein H（ompH）gene and the correlation with capsular type,the capsular typing and ompH gene of 44 strains of P.multocida were examined and anlalyzed by PCR method.The results showed that 22 strains were capsular type A,17 strains were capsular type B,5strains were identified as capsular type D.The open reading frames of ompH gene were from 1 002 to 1 056 bp.The predicted maturation proteins were composed of 313 to 331amino acids except the signal peptide including 20 amino acid residues in N terminal.The molecular weight of maturation proteins were from 33.83 to 36.46 ku.The alignment data indicated that the nucleotide sequence homologies were from 86.2% to100.0%,and the homology of amino acid were from 86.0% to 100.0%.The phylogenetic tree constructed by the ompH gene showed that capsular type A,B and D strains were in different branches.The sequence analysis results showed the ompH gene of P.multocidaisolated from swine had high homology among different serotypes.At the same time,there was a correlation between capsular type and the ompH gene.