γ-聚谷氨酸产生菌发酵培养基的响应面法优化研究

Optimization of fermentation medium for γ- PGA producing strain by response surface methodology

利用从纳豆中筛选得到的一株纳豆芽孢杆菌发酵生产γ-聚谷氨酸(γ-PGA)。在单因素优化实验的基础上,通过响应面法对发酵培养基进行优化,得到最佳培养基配方为蔗糖43.92 g/L、大豆蛋白胨7.00 g/L、谷氨酸钠46.32 g/L,γ-PGA产量由原来的7.253 g/L提高到11.794 g/L。

γ- polyglutamic acid was produced by fermentation with bacillus natto screened from natto. Optimization of the fermentation medium by response surface method on the basis of single factor experiments was studied. Results revealed that the optimal medium constituents were as follows ： sucrose 43.92 g/L, soy peptone 7.00 g/L, sodium glutamate 46.32 g/L. The production of γ- PGA increased from 7. 253 g/L to 11. 794 g/L.