慢性活动性乙肝患者胶原纤维、糜酶活性及羟脯氨酸含量表达研究

Chymase activity,collagen fiber and hydroxyproline content expression in patients with chronic active hepatitis b

目的:研究慢性活动性乙型肝炎患者胶原纤维、糜酶活性及羟脯氨酸含量表达及相关性。方法:选取100例慢性活动性乙型肝炎患者,行肝穿刺活检染色确定肝纤维化程度分期及炎症活动度分级,以直接测定胶原纤维相对含量,酶联免疫吸附法测定肝组织中糜酶活性,胃酶酸解法测定胶原纤维含量,荧光定量PCR技术测定HBV-DNA含量。结果:100例慢性活动性乙型肝炎患者肝组织胶原纤维含量为(0.13±0.05)mmol/L,靡酶活性为(24.77±15.08)ng/mg,羟脯氨酸含量为(20.84±7.46)μmol/L,HBV-DNA水平为(9.04±1.44)log10IU/mL;胶原纤维、靡酶活性及羟脯氨酸含量S1+S2期、S3+S4期高于S0期患者(P<0.05),S3+S4期高于S1+S2期(P<0.05);炎症活动度分级G3+G4级患者胶原纤维、靡酶活性及羟脯氨酸含量明显高于G1+G2级(P<0.05);胶原纤维、靡酶活性及羟脯氨酸含量与S分期、G分级间具有正相关性(P<0.05),胶原纤维、靡酶活性及羟脯氨酸含量、S分期、G分级与HBV-DNA水平间具有正相关性(P<0.05)。结论:慢性活动性乙型肝炎患者胶原纤维、糜酶活性及羟脯氨酸含量随着纤维化程度分期、炎症活动度分级而逐渐升高,其含量与HBV-DNA水平关系密切。

Objective： To explore chymase activity, collagen fiber and hydroxyproline content expression in patients with chronic active hepatitis b. Methods： A total of 100 patients with chronic active hepatitis b were selected, and had liver bi opsy to determine the degree of liver fibrosis stage and grade of inflammatory activity. The relative content of collagen fibers was measured, and chymase activity in liver tissue was measured by enzyme-linked immunosorbent assay. Content of collagen fiber was determined by gastric enzyme acid hydrolysis, and HBV - DNA content was determined by fluorescent quantitative PCR. Results： In liver tissue collagen fiber content was（0.13±0.05）mmol/L, chymase activity was （24.77± 15.08）ng/mg, hydroxyproline content was（20.84± 7.46）μmol/L, the HBV - DNA level was （9.04 ± 1.44） log10 IU/mL ; Collagen fibers, chymase activity and hydroxyproline content at stage S1 ＋ S2, S3 ＋S4 were higher than those at SO stage （P〈 0.05）, while these at S3 4- S4 stage were significantly higher than those at S1 ＋ S2 （P〈 0.05） ; Collagen fiber, chymase activity andhydroxyproline content at G3 ＋ G4 were significantly higher than these at G1 ＋ G2 （P〈 0.05） ; Collagen fibers, chymase activity and hydroxyproline content had positive correlation with S stage and grade of G （P〈 0.05）. Collagen fiber, ehymase activity and hydroxyproline content, S stage and grade of G had positive correlation with HBV - DNA levels （P〈 0.05）.Conclusions： Collagen fiber, chymase activity and hydroxyproline content in chronic active hepatitis b patients are increased with the increasing degree of fibrosis stage and grade of inflammatory activity, its content is closely associated with HBV- DNA level.