T淋巴细胞亚群和NK细胞对非霍奇金淋巴瘤患者疗效的预测价值

Effect of T lymphocyte and natural killer cell on patients with non-Hodgkin's lymphoma

目的:探讨T淋巴细胞亚群和NK细胞对非霍奇金淋巴瘤(NHL)患者疗效的预测价值。方法:选取本院收治的NHL患者120例为NHL组,另选同期体检人群60例作为对照组。所有NHL患者均单用化疗。于化疗前及化疗后1个月,检测T细胞及NK细胞水平。结果:化疗前,NHL组CD3+、CD4+细胞比例及CD4+/CD8+比值均明显低于对照组,CD8+、CD25+、CD4+CD25+Treg及NK细胞比例均明显高于对照组,差异具有统计学意义(P<0.05);化疗后,CD4+细胞比例、CD4+/CD8+比值较化疗前明显升高,CD4+CD25+Treg比例明显降低,与对照组比较差异具有统计学意义(P<0.05);不同临床分期及临床结局的NHL患者化疗前T细胞及NK细胞比例比较,差异具有统计学意义(P<0.05);临床分期越高、疗效越差,CD3+、CD4+细胞比例及CD4+/CD8+比值越低,CD8+、CD25+、CD4+CD25+Treg及NK细胞比例越高。结论:NHL患者外周血中T细胞及NK细胞与NHL存在密切关联,有望通过动态监测NHL患者外周血中T细胞及NK细胞水平来观察疾病发展及化疗的效果,指导临床治疗。

Objective： To investigate the effect of T lymphocyte and natural killer cell on patients with non-Hodgkin＇s lymphoma （NHL）. Methods： A total of 120 cases with NHL in our hospital were selected as the object and 60 people undergo- ing health examination were used as control group. All NHL patients just received chemotherapy. Before and 1 month after chemotherapy, T lymphocyte and natural killer （NK） cell level were determined. Results： Before chemotherapy, CD3＋ , CD4＋ cell ratio and CD4＋/ CD8＋ value of NHL group was significantly lower than that of control group, CD8＋, CD25＋ , CD4 CD25＋ Treg and NK cell ratio was significantly higher than that of control group （P〈0.05）. After chemotherapy, CD4＋ cell ratio and significantly increased and CD4＋ CD25＋ Treg ratio significantly decreased, also with significant difference compared with control group （P〈0.05）. For patients with different clinical phase and treatment outcome, T lymphocyte and natural kil let cell were significantly different. With the increasing of clinical phase and bad curative effect, CD3＋ ,CD4＋ ratio and CD4 ＋ /CD8＋ value decreased, CD8＋ , CD25＋ , CD4＋ CD25＋ Treg and NK cell ratio increased. （2onclusions.. There is closely relation- ship between T lymphocyte and natural killer cell with NHL. Detection of the level of T lymphocyte and natural killer cell in NHL patients was hopeful for predicting illness development and curative effect.