铜绿微囊藻、水华鱼腥藻对不同形态磷的摄取及生理响应

Uptake and Physiological Response at Different forms of Phosphorus by Microcystis aeruginosa and Anabaena flos-aquae

研究山仔水库2种水华蓝藻:铜绿微囊藻(Microcystis aeruginosa)、水华鱼腥藻(Anabaena flosaquae)在不同形态磷(4种无机磷和2种有机磷)条件下的生长效应.通过测定胞外总磷含量及碱性磷酸酶活性,比较两者对不同形态磷的摄取能力.结果显示,2种水华蓝藻对正磷酸盐(磷酸氢二钾、磷酸二氢钾)的吸收速度和碱性磷酸酶酶活均大于多聚磷酸盐(焦磷酸钠、聚磷酸钠)和有机磷盐(ATP、甘油磷酸钠);铜绿微囊藻磷吸收速率大于鱼腥藻,但水华鱼腥藻相应的比增长速率和碱性磷酸酶酶活性均大于微囊藻,表明正磷酸盐是水华蓝藻生长所需的最适磷盐;低磷条件下,水华鱼腥藻有可能成为优势藻.

To explore the effects of different types of phosphate on the growth of two water- bloom forming cyanobacteria （Microcystis aeruginosa and Anabaena flos-aquae. ）, four kinds of in- organic phosphorus and two kinds of organic phosphorus were used as phosphate sources in this ex- periment. To compare the uptake capacity of different forms of phosphorus, the content of intracel- lular total phosphorus and alkaline phosphatase activity were determined. The results showed that the phosphate uptake ability and alkaline phosphatase activity of the two cyanobacteria blooms in ortho- phosphate （dipotassinm hydrogen phosphate, monopotassium phosphate） greater than in poly phos- phate （sodium pyrophosphate, sodium tripolyphosphate ） and organic phosphorus （ATP, sodium glycerophosphate）. The growth rate and alkaline phosphatase activity of Anabaena flos-aquae in all phosphate group was higher than that of Microcystis aeruginosa, but lower ability of phosphate up- take than Microcystis aeruginosa. The phosphate uptake ability of Microcystis aeruginosa greater than Anabaena flos-aqaae, but less growth rate and alkaline phosphatase activity than Anabaena flos- aquae. It demonstrated that phosphate was the most suitable phosphorus salt for cyanobacteria blooms. Anabaena flos-aquae mav become the dominant algae under low P condition.