痰热清注射液对急性肺损伤大鼠的肺组织核因子-κB表达的影响

Effects of Tanreqing Injection on NF-κB of Acute Lung Injury Induced by Endotoxin in Rats Lung Tissue

目的通过建立急性肺损伤(ALI)大鼠模型,研究痰热清注射液对ALI治疗作用及可能机制,为其临床应用于感染所致的ALI提供理论参考。方法将SD大鼠随机分为正常对照组、模型2、4、6 h组、痰热清2、4、6 h组,每组8只。尾静脉注射脂多糖(LPS)造模,痰热清组在造模后从另一尾静脉注射痰热清注射液1 m L。在相应时间段取肺组织,观察肺病理形态学、组织损伤程度,通过免疫组化检测NF-κB p65的表达变化。结果(1)LPS模型组肺组织有明显的形态、病理学改变;各时间段模型组NF-κB p65的阳性指数值较正常对照组升高(P<0.01)。(2)痰热清2 h组NF-κB p65阳性指数与模型2 h组相比差异无统计学意义(P>0.05);痰热清4 h与6 h组阳性指数值较同时间点模型组明显降低(P<0.01),且与正常对照组无明显差异。结论痰热清注射液对ALI大鼠有保护作用,可以减轻其组织形态学改变,通过抑制肺组织NF-κB信号传导通路的活化,阻断炎症的级联式反应,从而减轻肺组织损伤,阻断ALI/ARDS的发生、发展。

Objective： To investigate the infects and mechanisms of Tanreqing Injection on lipopolysaccharide（LPS）-induced acute lung injury（ALI） in rats. Methods： 56 SD rats were randomly divided into 7 groups：normal control group,LPS model of 2 h,4 h,6 h group and Tanreqing Injection treatment of 2 h,4 h,6 h group.LPS was injected into tail vein in LPS model group and Tanreqing Injection treatment group. 1 m L Tanreqing Injection was injected into another tain veil in Tanreqing Injection treatment group. The rats were killed at each observation time point. The pathological changes of lung were observed,and the expression of NF-κB P65 in lung tissue were detected with immunohistochemisty. Results： Compared with the control group,the pathological changes of lung in LPS model group were significant. Compared with the control group,the expression of NF-κB P65 in lung tissue was significantly up-regulated in LPS model group.Compared with LPS model group,the expression of NF-κB p65 in lung tissue in Tanreqing Injection treatment group were significantly down-regulatedat 4 h and 6 h（P〈0.01）. Conclusion： Tanreqing Injection shows a protective effect on LPS-induced acute lung injury rats.The mechanism may be related to its ability of inhibiting the activation of the signal pathway of NF-κB and inflammatory cascade