摘要
研究不同浓度的碘化丙啶(PI)染色对细胞周期分布的影响。293T细胞经0、2、4、6Gy的X射线照射后,用浓度分别为5、10、20、50μg/ml的PI染色后,流式细胞仪检测细胞周期的分布情况。0、2、4、6Gy时,不同浓度PI染色时G1/G0期的比例变化如下:(35.90±0.17)至(37.65±0.42),(18.05±0.40)至(19.08±2.01),(4.24±1.31)至(5.89±1.08),(2.88±0.16)至(4.04±1.06),G2/M期的比例变化如下:(13.36±0.36)至(15.39±0.33),(54.56±0.34)至(58.98±1.46),(83.92±4.50)至(84.80±1.43),(92.29±1.56)至(92.89±1.49);S期的比例变化如下:(45.63±0.97)至(49.74±0.16),(22.45±0.35)至(27.04±0.36),(10.05±0.79)至(11.29±0.11),(3.71±1.06)至(4.99±1.01)。在同一照射剂量下,不管是G1/G0期、S期还是G2/M期,不同浓度PI染色对细胞周期分布没有明显差异(P>0.05);但不管5、10、20μg/ml还是50μg/ml的PI染色,随着照射剂量的增加,G2/M期细胞比例明显增加(P<0.01)。随着照射剂量的增加,G2/M期细胞比例明显增加;但对于同一照射剂量,不同浓度PI染色对细胞周期分布没有明显差异,做周期分布实验时选择5μg/ml的PI染色即可。
Study on the effect of different concentrations of propidium iodid in cell cycle distribution experiment. After 0,2,4,6Gy irradiation with X-ray and treated with propidium iodideof 5,10,20,50μg/ml, the cell cycle distri-bution of 293T cells was evaluatedby the flow cytometer. At 0, 2, 4, 6Gy, after dyed with propidium iodideof different concentrations, the percentage changes of G1/G0phase as follows:(35. 90±0. 17) ^(37. 65±0. 42),(18. 05±0. 40)^(19. 08±2. 01), (4. 24±1. 31) ^(5. 89±1. 08), (2. 88±0. 16) ^(4. 04±1. 06); the percentage changes of G2/M phase were:(13. 36±0. 36) ^(15. 39 ±0. 33),(54. 56 ±0. 34) ^(58. 98 ±1. 46), (83. 92 ±4. 50) ^(84. 80 ± 1. 43), (92. 29±1. 56) ^(92. 89±1. 49);the percentage changes of S phase as follows:(45. 63±0. 97) ^(49. 74± 0. 16), (22. 45±0. 35) ^(27. 04±0. 36), (10. 05±0. 79) ^(11. 29±0. 11), (3. 71±1. 06) ^(4. 99±1. 01). At the same dose, when stained with different concentration of PI, both G1/G0, S phase and G2/M phase, the percenta-ges were no significant difference (P〉0. 05);But regardless of 5, 10, 20μg/ml or 50μg/ml of PI, the cells propor-tion of G2/M phase increased significantly with the increase of irradiation dose ( P〈0. 01 ) . The G2/M phase cells pro-portion increased significantly with the increase of irradiation dose;But for the same dose, there was no significantdif-ference on the cell cycle distribution of different concentration of PI staining, So 5μg/ml of PI is appropriate for cell cycle distribution experiment.
出处
《激光杂志》
CAS
北大核心
2015年第1期144-147,共4页
Laser Journal
基金
国家自然科学基金(NO.81302383)资助课题