高效液相色谱法同时测定暑热宁口服液中葛根素和绿原酸的含量

High Performance Liquid Chromatography and Determination of Puerarin in Shurening Oral Liquid and Content of Chlorogenic Acid

目的：建立不同检测波长下同时测定暑热宁口服液中葛根素和绿原酸液相色谱方法。方法：采用高效液相色谱法,色谱柱：Xterra MS18 5μm 46×250nm;流动相：乙睛-0.3%磷酸溶液（10∶90）;流速：1.0 m L/min;检测波长：250nm、327 nm;柱温：35℃;进样量20μL。结果：葛根素在进样量为1.2824-12.824μg内与峰面积线性关系良好,Y=9.70e﹢007X＋1.73e＋005（r=0.9999）,加样回收率（n=9）为95.6%-99.6%,RSD为1.44%。绿原酸在进样量为1.360-13.60μg内与峰面积线性关系良好,Y=3.20e﹢006X＋5.60e﹢005（r=0.9997）,加样回收率（n=9）为99.2%-103.4%,RSD为1.9%。结论：该方法简便快速,结果准确,可以有效控制暑热宁口服液中葛根素和绿原酸的含量。

Objective： To establish different wavelength detections and determinations of puerarin in Shurening Oral Liquid and chlorogenic acid chromatography method. Methods： By high performance liquid chromatography,chromatography column： Xterra MS18（ 5 μm,46 nm × 250 nm）. Mobile phase： beyes-0. 3% phosphoric acid solution（ 10： 90）; flow rate： 1. 0 m L / min; detection wavelength 250 nm,327 nm; column temperature： 35 ℃; sample quantity： 20 μL. Results： Puerarin in sample quantity from1. 2824 to 1. 2824 μg had a good linear relationship with the peak area in Y = 9. 70 e ﹢ 007 X ＋ 1. 73 e ﹢ 005（ r = 0. 9999）. The sample recovery rate was 95. 6%-99. 6%（ n = 9） and RSD was 1. 44%. Of chlorogenic acid in sample quantity,from 1. 360 to1. 360 μg it had good linear relationship with the peak area in Y = 3. 20 e ﹢ 006 X ＋ 5. 60 e ＋ 005,（ r = 0. 9997） and the sample recovery rate（ n = 9） was 99. 2%-103. 4% and RSD was 1. 9%. Conclusion： This method is simple and rapid,accurate,and can effectively control the Shurening Oral Liquid in puerarin and the content of chlorogenic acid.