南方红豆杉内生真菌的分离及抗菌活性筛选研究

Isolation of Endophytic Fungi from Taxus chinensis var. mairei Cheng et L. K and Its Antimicrobial Activity

目的对我国广西产的野生南方红豆杉内生真菌进行分离,并研究其抗菌活性,旨在筛选具有良好抗菌活性的真菌菌株,以寻找新型抗菌物质。方法以组织块分离法获得内生真菌,并以金黄色葡萄球菌、大肠杆菌和白色念珠菌为实验菌株,用琼脂块法和纸片扩散法对分离的菌株进行抗菌活性筛选。结果从南方红豆杉根、茎、叶中共分离到34株内生真菌,其中有8株真菌对至少1种实验菌株有抗菌活性,占总分离菌株的23.53%。结论南方红豆杉内生真菌种类多样,而且存在着丰富的抗菌天然活性物质,这可为寻找新型抗菌物质提供可利用的候选菌株。

OBJECTIVE To isolate the endophytic fungi from medicinal plant Taxus chinensis var. mairei Cheng et L. K in Guangxi Zhuang autonomous region, and investigate its antimicrobial activity. METHODS Endophytic fungi were isolated by the tissue separation method. Staphylococcus aureus, Escherichia coll, and Candida albicans were used as the indicators for human pathogenic microbes. Antimicrobial tests of the isolated endophytic fungi were carried out by agar block and disc diffusion. RESULTS Among 34 strains of endophytic fungi isolated from roots, stems, and leaves of T. chinensis var. mairei, eight strains （23.53 % of the total isolated strains） showed antimicrobial activity against one or more of three human pathogenic microbes. CONCLUSION Diverse antimicrobial natural products exist in endophytic fungi from T. chinensis var. mairei, which can be the resources for new antimicrobial products.