摘要
本研究建立了高效液相色谱(HPLC)同时测定预混合饲料中维生素c、维生素B:及其磷酸盐形式维生素C-2-磷酸酯(VCP)、维生素琰-5-磷酸钠(VB,P)的方法。预混合饲料用1.5mmol/L四丁基硫酸氢铵缓冲溶液提取后,进行HPLC检测。采用ZorbaXEclipsePlusC18色谱柱(250rTlfnX4.6mm,5μm),以1.5mmol/L四丁基硫酸氢铵缓冲溶液(pH=3.0)与甲醇作为流动相,梯度淋洗。在此色谱条件下,维生素c、维生素B。VCP和VB2P的线性关系良好,检出浓度分别为3、1、1、3mg/kg,定量限为10、5、5、10mg/kg。应用该法测定了预混合饲料中维生素c、维生素B2、VCP和VB2P,平均回收率为81.5%~106%,相对标准偏差(RSD)均小于10%。
A high performance liquid chromatographic (HPLC) method with ultraviolet detection was established to determine vitamin C,vitamin B2 and its phosphate (vitamin C-2- phosphate (VCP)and vitamin B2-5- phosphate (VB2P))in additive premix simultaneously.Additive premix were extracted by 1.5 mmol/L tetrabutylammonium hydroxide,then detected by HPLC.A Zorbax Eclipse Plus-C18 column (250 minx 4,6 ram,5μm)was used in the analysis.The separation was ear- ried out using the mobile phase consisting of 1.5 mmol/L tetrabutylammonium hydroxide (pH 3.0)-methanol at a flow rate of 1.0 mL/min.The eluates were monitored by the ultraviolet detection setting at 255 nm.The linearities were from 1 mg/L to 500 mg/L,for vitamin C ,vitamin B2,VCP,VB2P.The detection limit was 3,1,1,3 mg/kg,and 10,5,5,10 mg/kg for vita- min C,vitamin B2,VCP,VB2P.The mean recoveries were from 81.5% to 106% ,precision were less than 10% ,respectively. The method was very reliable for the simultaneous determination of vitamin C, vitamin B2, VCP,VB2P in additive premix.
出处
《中国饲料》
北大核心
2015年第2期34-37,共4页
China Feed
关键词
高效液相色谱
维生素C
维生素B2
磷酸盐
预混合饲料
high performance liquid chromatography
vitamin C
vitamin B2
phosphate
additive premix