仿刺参糖氨聚糖的特异性免疫调节作用的研究

The research of Glycosaminoglycan fromApostichopus Japonius on specific immune adjustment

目的研究了仿刺参糖氨聚糖（HGAG）对小鼠特异性免疫功能的影响,探讨其对小鼠的特异性免疫的作用。方法不同浓度的HGAG与小鼠脾细胞悬液共培养44h,MTT法检测;将昆明种小鼠32只,分为4组,每组8只,空白组注射生理盐水,HGAG组（0.1,1,10mg·kg^-1）,每天给药,第1天将全部小鼠腹腔注射压积SRBC,4d后观察小鼠足趾肿胀程度;5d后小鼠摘眼球取血,540nm测OD值,计算半数溶血值（HC50）;将小鼠处死,取脾制成单细胞悬液经培养,413nm测OD值,检测抗体生成细胞数。结果 HGAG在0.05-25μg·mL^-1范围内能明显促进小鼠脾淋巴细胞的增殖,与空白组相比具有显著性差异（P〈0.05）,在0.5-10μg·mL^-1范围内,活性优于阳性组（P〈0.05）,在HGAG浓度为1μg·mL^-1时促进增殖作用最为明显;HGAG在低、中、高剂量组均能促进小鼠迟发性变态反应,且随着剂量浓度的增加而增高（P〈0.05）;显著提高血清溶血素及抗体生成细胞数（P〈0.05）。HGAG对正常小鼠的细胞免疫、体液免疫均有明显的增强作用,进一步说明HGAG在特异性免疫方面具有一定的调节作用。

objective To investigate the influence of Glycosaminoglycan from Apostichopus Japonius （HGAG） on specific immunological function in mice, and explore the effects on specific immunity in mice. Methods Different concentrations of HGAG were added into the mouse spleen cell suspension for 44 h, and measured the absorbance by MTT method. The mice were randomly divided into blank group, HGAG groups （0. 1, 1, 10 mg ·kg^-1）, with 8 mice per group, injected with physiological saline and the corresponding dose HGAG daily, respectively. The mice of the thickness of the toes were measured after sensitization by intraperitoneal injection of deposited SRBC. After 5 d, mice eyeball blood was picked and OD values were measured in 540 nm, then calculated the half hemolysis value （HC50）. Single cell suspension from the killed mice was measured in 413 nm after culturing for detec ring antibody generating cell number. Results HGAG could obviously promote the proliferation of mice spleen lymphocyte in 0.05-25 μg · mL^-1 , compared with the blank group （P〈0.05）. HGAG showed more significant promotion effect than the positive control in 0. 5--10μg · mL^-1 （P〈0. 05）, and the proliferative promotion effect reached the highest in 1 μg · mL^-1. HGAG could accelerate the mice de layed type hypersensitivity reaction in low, medium and high dose group, and DTH would rise with the increase of dose concentration （P〈0.05）. Serum hemolysin level and antibody generating ceils number could enhance significantly （P〈0. 05）. The cellular immunity and humoral immunity of normal mice could be obviously enhanced by HGAG, which illustrated certain regulative effects on the specific immunity further.