抗松材线虫病赤松组培苗菌根化研究

Mycorrhization of tissue-cultured plantlets of nematode-resistant Pinus densiflora

为提高抗病赤松组培苗的生根率及生根质量,以抗松材线虫病赤松瓶内组培苗为试验材料,比较了菌根真菌发酵液接种、菌块接种及发酵液浸根3种方式对组培苗生根率及移栽成活率的影响。结果表明:3种接菌方式对赤松生根率均无显著影响,而对移栽成活率有显著影响。对赤松组培苗接种美味牛肝菌(Be)、黄色须腹菌(Rl)和彩色豆马勃(Pt2)3种菌根真菌,发现3种菌根真菌均能与组培苗形成菌根,但菌根化程度及菌根的形态表现出一定差异,Pt2与赤松组培苗形成的菌根状态最好,且移栽成活率较高,是适合赤松组培苗菌根化的优良菌根真菌。

To increase the rooting rate and improve the quality of adventitious root system, mycorrhization of nematodecultured plantlets of nematode-resistant Pinus densiflora was studies. Three inoculation methods were compared. The resuits showed that there was no significant effect on rooting rate after inoculating ectomycorrhizal fungi with different methods at the root elongation stage, but the survival rate was affected significantly. To some extent, inoculation of mycorrhi- zal fungi could improve the survival of tissue-cultured plantlets. Significant difference has been ohsererved at the degree of mycorrhization and morphological characteristics after inoculating with Rhizopogen luteous （ R1）, Pisolithus tinctorius （Pt2） and Boletus edulis （Be）. Pt2 was the most compatible ectomycorrhizal partner for P. densiflora. The survival rate of plantlets inoculated with Pt2 was higher than those inoculated with R1 and Be.