摘要
【目的】研究杭州地区耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus,MRSA)的基因型别,探讨MRSA菌株流行变化趋势及进化特点,为该地区MRSA的进一步防治提供科学依据。【方法】对86株MRSA进行葡萄球菌盒式染色体SCCmec基因、spa基因分型,并开展多位点序列分型(Multi-locus sequence typing,MLST),与国际上MRSA的流行型别进行比较,分析进化关系。【结果】86株MRSA共发现13个spa型(以t311型为主,占48.8%;其次为t6418型,占11.6%);MLST分型共发现9个ST型(以ST5为主,占59.3%;其次为ST239,占16.3%),经e BURST软件分析它们属于4个群(Group 1、Group 6、Group 8、Group 12)和8种克隆复合体(CC1、CC5、CC630、CC20、CC59、CC88、CC239、CC573);SCCmec基因分型以SCCmecⅡ型为主,占61.6%;其次为SCCmec III型,占22%;5株社区相关性MRSA(SCCmec-Ⅳ型)。其中第一流行克隆型为SCCmec-Ⅱ-ST5-t311-CC5(占47.7%)、其次为SCCmec-III-ST239-t030/t037-CC239(占12.8%)。【结论】SCCmec-Ⅱ-ST5-t311为杭州地区当前流行菌株;CA-MRSA菌株的出现,提示MRSA菌株有由医院向社区播散的趋势;此外,对新发展了单位点变体的菌株(SCCmec-Ⅰ-ST1921-t164-CC20和SCCmec-Ⅳ-ST965-t062-CC5),应加强重视。
[Objective] To analyze the genotype of Methicillin-resistant Staphylococcus aureus （MRSA）, discover the epidemiology and evolutionary pattern of MRSA and provide a scientific basis for prevention and control of MRSA in Hangzhou area. [Methods] In total 86 MRSA strains were characterized by spa typing, multi-locus sequence typing （MLST）, staphylococcal cassette chromosome mec （SCCrnec） typing. Results were compared with prevalent genotypes of MRSA reported worldwide to analyze the evolutionary relationships. [Results] The 86 MRSA isolates were classified into 13 spa types and 9 STs, which were further categorized into 4 groups and 8 clonal complex. The SCCmecIIwas the predominant genotype （61.6%）, followed by SCCmec-Ⅲ （22%）, 5 strains of CA-MRSA （SCCmeclV）. SCCmec-Ⅱ-STS-t311-CC5 and SCCmec-Ⅲ-ST239-tO30/t037-CC239 were the main clones, representing for 47.7% and 12.8%, respectively. [Conclusion] SCCmec-Ⅱ-ST5-t311 was the most prevalent strain of MRSA in Hangzhou area. The emergence of CA-MRSA strains suggested that MRSA has spread from hospitals to community in Hangzhou. In addition, the strains （SCCmec-I-ST1921-t164-CC20 and SCCmec-IV-ST965- t062-CC5） developed a new variant strain that needs more attention.
出处
《微生物学通报》
CAS
CSCD
北大核心
2015年第1期110-116,共7页
Microbiology China
