转基因小麦抗旱性鉴定及相关指标灰色关联度分析

Characterization of drought resistance in transgenic wheat lines and grey correlation analyses of related traits

以9份转 ABP9基因春小麦株系UC4－1～UC4－9及其受体亲本宁春4号为材料，在雨养和灌溉条件下，对这些材料的主要农艺性状和部分生化指标进行了测定，采用隶属函数值法和抗旱性度量（D）值法对其抗旱性进行了综合评价，并利用灰色关联度法对相关抗旱性状与抗旱指数之间的关联度进行了分析评价。结果表明：6份转基因小麦的抗旱性较受体亲本宁春4号增强，3份材料的抗旱性较非转基因春小麦品种定西35增强；各农艺性状和生化指标与抗旱指数的关联度从高到低依次分别为株高、单穗粒重、穗长、SOD活性、穗粒数、结实小穗数、有效分蘖数、POD活性、千粒重、脯氨酸含量、MDA含量。

A total of 11 traits ,including 7 agronomic traits and 4 biochemical traits ,was measured in nine ABP9-transgenic spring wheat lines and their donor parent Ningchun 4 that were grown in rain-fed （drought stress ） and well-wa-tered environments .Drought resistance capabilities of these materials were evaluated comprehensively by employing the methods of subordinate function and drought-resistance measure value （ D value ） . Correlation degrees between these traits and drought resistance indexes were analyzed and evaluated using grey relational grade analysis method .The results showed that the drought resistance of the 6 transgenic wheat lines was better than that of their donor parent Ningchun 4 . In particular ,3 lines even exhibited stronger drought resistance than a non-transgenic spring wheat cultivar Dingxi 35 , which was commonly used as a control for testing drought resistance of drought-land spring wheat in the northwest Chi-na .Results of grey relational grade analyses indicated that the correlation degrees between these traits and drought resis-tance indexes were ranked from high to low in the following order：plant height ,single spike weight ,spike length ,SOD activity ,number of kernel per spike ,effective tillers ,POD activity ,1000-kernel weight ,proline content ,and then MDA content .