利用siRNA高通量测序技术检测烟草病毒

Detection for RNA viruses in tobacco by High-throughput sequencing of siRNA

烟草是我国的主要经济作物之一，病毒病是烟草生产上的重要病害。常见的烟草病毒主要有马铃薯Y病毒（Potato virus Y，PVY）、黄瓜花叶病毒（Cucumber mosaic virus，CMV）、烟草花叶病毒（Tobacco mosaic virus，TMV）、烟草脉带花叶病毒（Tobacco vein banding mosaic virus，TVMBV）等。此外，近年来在烟草上还发生一些新的病毒病害，

Plant obtains immunity against RNA virus through small interference RNA （siRNA） mechanism, we adopt next-generation sequencing technology to perform high throughput sequencing of tabacco siRNA and screening for RNA virus sequences with bioinformatics methods. We found that PVY, CMV, TMV, TVBMV and other virus genomic information in Anhui Province. This research established a new strategy to find naturally RNA viruses in tobacco, and this strategy could be applied to virus surveillance in tobacco.