三七总皂苷调节哮喘小鼠气道炎症的免疫分子机制研究

Research of molecular immune mechanism of PNS in alleviating asthmatic mouse airway inflammation lesions

目的观察三七总皂苷(PNS)对哮喘小鼠气道炎症的疗效及对免疫细胞Th1/Th2平衡的调节作用,从免疫平衡的角度寻找其作用靶点,探讨PNS防治支气管哮喘的可能作用机制。方法将SPF级BALB/c雌性小鼠50只随机分成正常对照组、哮喘模型组及PNS低、中、高剂量组。以卵清白蛋白(OVA)腹腔注射及雾化激发法构建哮喘模型。观察小鼠肺组织病理变化,用酶联免疫吸附法(ELISA)检测各组小鼠血清中IL-4、IFN-γ的变化。结果 PNS低、中、高剂量组哮喘症状有所减轻,肺组织炎性细胞浸润减轻,且小鼠血清中IFN-γ的表达具有升高趋势,以低、中剂量组效果明显(P<0.05和P<0.01),PNS中剂量组和PNS高剂量组血清IL-4浓度明显降低(P<0.01)。结论 PNS可减轻哮喘小鼠肺组织炎性细胞浸润,减少气道上皮黏液分泌,改善气道炎症状态。PNS防治哮喘的机制之一可能是通过上调哮喘小鼠IFN-γ的分泌,抑制IL-4的分泌,进而促进Th0向Th1分化,抑制Th0向Th2分化,恢复Th1/Th2平衡状态。

Objective It is to observe the effects of Panax notoginseng saponin（ PNS） on the airway inflammation in asthmatic mice and Th1 / Th2 related cytokines,and to fine the target from the point of immunologic balance,thus to explore the possible mechanism of PNS in preventing asthma. Methods 50 SPF level BALB / c female mice were divided into normal control group,asthma model group,PNS low,medium and high dose groups. The asthma models were established by OVA intraperitoneal injection and atomization inspired method. The pathological changes in the lung tissue of mice were observed,and the changes of serum IL- 4 and IFN- γ were detected by enzyme- linked immunosorbent assay（ ELISA）. Results Asthma symptoms and inflammatory cell infiltration were relieved in PNS low,medium and high dose groups,furthermore the expression of IFN- γ in serum of the mice had a increase trend,especially in low and medium dose groups（ P〈0. 05 or P〈0. 01）. The serum concentration of IL- 4 decreased significantly in PNS medium and high dose groups（ P〈0. 01）. Conclusion PNS can relieve infiltration of inflammatory cells in lung tissue in the mice with asthma,reduce airway epithelial mucus secretion,improve airway inflammation. One of the mechanisms for PNS prevention of asthma in asthmatic mice may be by up-regulating secretion of IFN-γ,inhibiting the secretion of IL- 4,thus contributing to Th0 to Th1 differentiation,inhibit Th0 to Th2,restore Th1 /Th2 balance.