冠心病慢性心力衰竭患者外周血CD^(4+)CD^(25+)Foxp^(3+)Treg细胞的研究

Study of Peripheral Blood CD^(4+)CD^(25+)Foxp^(3+)Treg Cell in Patients with Coronary Heart Disease and Chronic Heart Failure

目的测定冠心病慢性心力衰竭患者的免疫功能及血浆N端脑钠肽前体(NT-proBNP)水平,评价冠心病慢性心力衰竭的免疫激活状态。方法收集2013年8月—2014年2月,中国中医科学院广安门医院心内科的住院患者125例,其中慢性心力衰竭患者93例,非心力衰竭患者32例。采用化学发光免疫方法测定血浆NT-proBNP水平,流式细胞技术测定外周血CD4+CD25+Foxp3+Treg细胞比例。结果冠心病慢性心力衰竭患者血浆NT-proBNP高于冠心病非心衰组,而外周血CD4+CD25+Foxp3+Treg细胞比例低于非心力衰竭组。结论冠心病慢性心力衰竭患者存在免疫激活状态,且血浆NT-proBNP水平随NYHA心功能分级增加而升高。

Objective To observe the immune function and plasma N-terminal pro-B-type natriuretic peptide（NT-proBNP）levels,and to evaluate the state of immune activation in in patients with coronary heart disease（CHD）and chronic heart failure（CHF）.Methods From August 2013 to February 2014 in department of cardiology of Guang＇anmen hospital of traditional Chinese academy,a total of 125 in patients,of whom 93 patients with CHF,32 patients with non-heart failure were chosen.The level of plasma NT-proBNP was determined by chemiluminescence immunoassay method.The peripheral blood CD4＋CD25＋Foxp3＋Treg cell ratio was measured by flow cytometry.Results The levels of plasma NT-proBNP in patients with CHF were higher than that in patients with non heart failure.The peripheral blood CD4＋CD25＋Foxp3＋Treg cells in patients with CHF was lower than that in patients with non heart failure.Conclusion There is the presence of immune activation state in patients with CHF and the levels of plasma NT-proBNP increase with increasing New York Heart Association（NYHA）functional class.