盐霉素钠纳米脂质体的制备及表征

Preparation and characterization of salinomycin sodium loaded nano liposomes

目的制备并表征盐霉素钠纳米脂质体(SLN)。方法采用薄膜分散法制备盐霉素钠纳米脂质体,通过调节脂质体中胆固醇比例,以盐霉素钠包封率为评价指标,筛选盐霉素钠纳米脂质体的优化处方。结果透射电镜显示盐霉素钠纳米脂质体形态圆整,分散性良好,激光粒度仪显示盐霉素钠纳米脂质体平均粒径为99.0nm,Zeta电位为-33.5mV,包封率为85.7%,载药量为6.7%。通过脂质体包裹,盐霉素钠在水中的最高浓度可提高15倍,并证明其具有一定缓释效果。结论笔者得到了粒径大小在100nm左右,形态均一,包封率和载药量较高的盐霉素钠纳米脂质体,为进一步测定其杀伤肿瘤活性奠定了坚实的制剂学基础。

Objective To prepare and characterize salinomycin sodiumloaded nano liposomes（SLN） .Methods The nano liposomes were prepared by a thinfilm dispersion method .The formula of SLN was optimized by regulating the cholesterol ratio of the nano liposomes ,using the encapsulation efficacy （EE） of SLN as the primary outcome measure .Results Transmission electron microscope （TEM） showed that SLN was round and had a good dispersion .Dynamic laser scatter （DLS） showed that SLN was of a desired size of 99 nm ,and zeta potential of -33 .5 mV .EE of SLN was 85 .7% and drug loading of 6 .7% .According to the formulation of nano liposomes ,the concentration of salinomycin sodium in water was greatly improved by 15 folds .Additionally ,the nano liposomes were observed to exhibit sustained release characteristics .Conclusion Salinomycin sodiumloaded nanoliposomes of a desired size of about 100 nm were obtained ,which were well dispersion ,and high EE and drug loading .Solid pharmaceutics foundation for the activity examination of SLN was provided in this research .