摘要
目的：筛选优化沙棘粉中儿茶素的提取方法,并用HPLC测定其含量。方法：以均匀设计法优化提取条件,采用Agilent ZORBAX SB-C18柱,以甲醇—乙酸溶液（用乙酸调p H值至3.2）（25：75）为流动相,流速1 mL/min;柱温25℃,检测波长280nm。结果：优选出的最佳提取条件：甲醇浓度为74.0%,水浴温度为70℃,超声时间为30min。儿茶素在0.50-10.0μg/m L浓度范围内线性良好,相关系数（r）为0.9999,平均回收率为97.25%（RSD=1.77%）。结论：本试验建立的含量测定方法操作简便、重现性好且具有较强的专属性。
Objective： Screening methods to optimize the extraction of catechins sea buckthorn powder, and determine its content by HPLC. Methods： With uniform design to optimize ultrasonic extraction conditions, using Agilent ZORBAX SB- C l 8 column with methanol - acetic acid solution （pH value adjusted to 3.2 with acetic acid） （25：75） as the mobile phase, flow rate 1 mL / min ; column temperature was 25 ℃ The detection wavelength 280nm. Results： The optimum extraction con- ditions optimized concentration of 74.0% for methanol, water bath temperature of 70 ℃, ultrasonic time of 30min. Catechin at 0.50 - 10.0μg / mL concentration range good linearity, the correlation coefficient （r） was 0.9999, the average recovery was 97.25% （ RSD=1.77% ）. Conclusion： Method for the determination of the trial established simple, reproducible and has a strong specificity.
出处
《福建分析测试》
CAS
2015年第1期46-49,共4页
Fujian Analysis & Testing
基金
国家质检总局科技计划项目
计划编号:2013IK172
